Protein Info for HER17_RS14505 in Pectobacterium carotovorum WPP14

Annotation: NADP-dependent phosphogluconate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03446: NAD_binding_2
  • amino acids 5 to 173 (169 residues), 173.2 bits, E=6.6e-55
• TIGR00873: 6-phosphogluconate dehydrogenase (decarboxylating)
  • amino acids 5 to 467 (463 residues), 763.7 bits, E=3.6e-234
• PF00393: 6PGD
  • amino acids 178 to 466 (289 residues), 452.6 bits, E=9.5e-140

Best Hits

Swiss-Prot: 88% identical to 6PGD_SALTY: 6-phosphogluconate dehydrogenase, decarboxylating (gnd) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00033, 6-phosphogluconate dehydrogenase [EC: 1.1.1.44] (inferred from 99% identity to pct:PC1_1320)

MetaCyc: 88% identical to 6-phosphogluconate dehydrogenase, decarboxylating (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydrogenase (decarboxylating). [EC: 1.1.1.44]

Predicted SEED Role

"6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)" in subsystem Pentose phosphate pathway (EC 1.1.1.44)

MetaCyc Pathways

• superpathway of glucose and xylose degradation (16/17 steps found)
• pentose phosphate pathway (8/8 steps found)
• pentose phosphate pathway (oxidative branch) I (3/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Glutathione metabolism
• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.44

Protein Sequence (468 amino acids)

>HER17_RS14505 NADP-dependent phosphogluconate dehydrogenase (Pectobacterium carotovorum WPP14)
MSKQQIGVVGMAVMGRNLALNIESRGYSVSIFNRSSDKTDEVIAENPGKKLVPSYTVEEF
VESLEKPRRILLMVKAGEATDKTIESLKPYLEKGDILIDGGNTFYKDTIRRNRELSAEGF
NFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILEQIAARADGEACVTYIGADGAGHY
VKMVHNGIEYGDMQLIAEAYALLKQALNLNNDELAATFSEWNKGELSSYLIEITADIFKK
KDEDGNYLVDVILDEAANKGTGKWTSQSSLDLGEPLSLITESVFARYLSSLKDQRVAASK
VLTGPTAQAFGGDKAEFVEKVRRALYLGKIVSYAQGFSQLKAASDENNWDLNYGEIAKIF
RAGCIIRAQFLQKITDAYEQKADIANLLLAPYFKKVADEYQQALRDVVSYAVQQGIPTPT
FSAAIAYYDSYRSAVLPANLIQAQRDYFGAHTYKRTDKEGVFHTEWLE