Protein Info for HER17_RS12945 in Pectobacterium carotovorum WPP14

Annotation: Bcr/CflA family multidrug efflux MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 transmembrane" amino acids 9 to 26 (18 residues), see Phobius details amino acids 46 to 65 (20 residues), see Phobius details amino acids 77 to 96 (20 residues), see Phobius details amino acids 102 to 123 (22 residues), see Phobius details amino acids 135 to 159 (25 residues), see Phobius details amino acids 165 to 185 (21 residues), see Phobius details amino acids 216 to 239 (24 residues), see Phobius details amino acids 251 to 269 (19 residues), see Phobius details amino acids 280 to 302 (23 residues), see Phobius details amino acids 304 to 304 (1 residues), see Phobius details amino acids 310 to 332 (23 residues), see Phobius details amino acids 344 to 364 (21 residues), see Phobius details amino acids 370 to 390 (21 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 8 to 390 (383 residues), 458.5 bits, see alignment E=1.2e-141 PF07690: MFS_1" amino acids 16 to 358 (343 residues), 167.3 bits, see alignment E=4.6e-53 PF00083: Sugar_tr" amino acids 48 to 184 (137 residues), 31.2 bits, see alignment E=1.1e-11

Best Hits

Swiss-Prot: 71% identical to BCR_ECOLI: Bicyclomycin resistance protein (bcr) from Escherichia coli (strain K12)

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 98% identity to pct:PC1_1640)

MetaCyc: 71% identical to multidrug efflux pump Bcr (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-340; TRANS-RXN-44

Predicted SEED Role

"MFS family multidrug transport protein, bicyclomycin resistance protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (396 amino acids)

>HER17_RS12945 Bcr/CflA family multidrug efflux MFS transporter (Pectobacterium carotovorum WPP14)
MQLRRSSHFGLIFILGLISMLMPLAIDMYLPALPTIANEFGVGDGHVQMTLSTYVLGFAI
GQMFYGPMADSLGRKPVILGGTLVFAFAGVACALAQTVEQLIYMRFLHGVSAAAAAVVIN
ALMRDMFSRDDFSRMMSFVVLVMTVAPLIAPIAGGWLLLWFSWHSIFWTISGAAFLASAL
IFFFIKETLPPAKRQKFHLRTTIGNFAILFRHKRVFSYMVASGLSFCGMFSFLSAGPFVY
INLYGVSPQNFGYYFALNVVFLFVVTLFNSRNVRRLGALAMFRAGLVIQFAMGIWLVIVC
LFDLGFLPLVFGVALFVGCIAMVASNAMAVILDDFPHMAGTASSLAGTLRFGLGAMVGGI
LSLASFNSAWPMVLSMALCSIGAFLLYLYATRSPAS