Protein Info for HER17_RS12855 in Pectobacterium carotovorum WPP14

Annotation: PTS fructose transporter subunit IIBC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 563 transmembrane" amino acids 237 to 257 (21 residues), see Phobius details amino acids 269 to 292 (24 residues), see Phobius details amino acids 304 to 330 (27 residues), see Phobius details amino acids 350 to 370 (21 residues), see Phobius details amino acids 382 to 404 (23 residues), see Phobius details amino acids 416 to 444 (29 residues), see Phobius details amino acids 465 to 483 (19 residues), see Phobius details amino acids 490 to 512 (23 residues), see Phobius details amino acids 522 to 552 (31 residues), see Phobius details TIGR00829: PTS system, Fru family, IIB component" amino acids 106 to 189 (84 residues), 131.2 bits, see alignment E=1.3e-42 PF02302: PTS_IIB" amino acids 107 to 195 (89 residues), 71.9 bits, see alignment E=5.9e-24 TIGR01427: PTS system, Fru family, IIC component" amino acids 219 to 553 (335 residues), 467.1 bits, see alignment E=4.2e-144 PF02378: PTS_EIIC" amino acids 234 to 497 (264 residues), 67.1 bits, see alignment E=1.5e-22

Best Hits

Swiss-Prot: 75% identical to PTFBC_ECOLI: PTS system fructose-specific EIIB'BC component (fruA) from Escherichia coli (strain K12)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 98% identity to pwa:Pecwa_1926)

MetaCyc: 75% identical to fructose-specific PTS multiphosphoryl transfer protein FruA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"PTS system, fructose-specific IIB component (EC 2.7.1.69) / PTS system, fructose-specific IIC component (EC 2.7.1.69)" in subsystem Fructose utilization (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (563 amino acids)

>HER17_RS12855 PTS fructose transporter subunit IIBC (Pectobacterium carotovorum WPP14)
MKTLLILDKSLGLARSQLVKNLLGAAAAKAGVTFTEQANDAELAIVLGASAAADSALNGK
QVYVGDIELAVSQPDAFLAKAQAEATVYQAAASAPVQQPAAAKRIVAITACPTGVAHTFM
AAEAIESEAKKRGWWVKVETRGSVGAGNAITLEEVEQADLVIVAADIEVDLAKFAGKKMY
RTSTGLALKKTAQELDKAQAEAKVYQPSGQASASSASESGQKGGAGPYRHLLTGVSYMLP
MVVAGGLCIALSFVFGIEAFKQEGTLAAALMKIGGGSAFALMVPVLAGYIAFSIADRPGL
TPGLVGGMLAVSTGAGFLGGIIAGFLAGYIARAINNKLILPQSLTALKPILIIPLFATLI
TGLIMIYVVGTPVAKILTGLTGWLQSMGTANAVILGAILGAMMCTDMGGPVNKVAYVFGT
TLLSSQIYAPMAAVMAAGMVPPLAMGLATVLASKKFNPTEREGGKAAFVLGLCFISEGAI
PYAARDPMRVLPCCIIGGALTGALSMAVGAKLMAPHGGLFVLLIPGAITPVIGYLLAIIA
GTAVAGVLYALLKRSDEQLAKVA