Protein Info for HER17_RS11800 in Pectobacterium carotovorum WPP14

Annotation: murein DD-endopeptidase MepM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details PF08525: OapA_N" amino acids 12 to 38 (27 residues), 34.7 bits, see alignment (E = 3.1e-12) PF04225: OapA" amino acids 97 to 160 (64 residues), 28.4 bits, see alignment E=3.6e-10 PF01476: LysM" amino acids 99 to 141 (43 residues), 29.3 bits, see alignment 1.7e-10 PF19425: Csd3_N2" amino acids 180 to 301 (122 residues), 219.5 bits, see alignment E=2.2e-69 PF01551: Peptidase_M23" amino acids 313 to 407 (95 residues), 124.9 bits, see alignment E=3.2e-40

Best Hits

Swiss-Prot: 75% identical to MEPM_ECOL6: Murein DD-endopeptidase MepM (mepM) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 98% identity to pct:PC1_1828)

MetaCyc: 75% identical to peptidoglycan endopeptidase MepM (Escherichia coli K-12 substr. MG1655)
3.4.-.-; 3.4.-.-

Predicted SEED Role

"Cell wall endopeptidase, family M23/M37"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>HER17_RS11800 murein DD-endopeptidase MepM (Pectobacterium carotovorum WPP14)
MQQIVRTIALAYNSLPRPHRVMLGSLTVVTLAVAVWRPFVYPHVDDAPVIVKDIDLETSQ
LRTLTPEASEPIDQPSPDDEIPQDELDDKVADEGGVHEYVVSTGDTLSSILTQYGIDIAD
VTQLAEQNKDLRNLKIGQQISWALAENGDLQSLTWQMSRRETRIYERVGADFKERIETLK
GEWRNSVLNGRVSGSFVSSAKNAGLTSTEVREVIKALQWQLDFRKLRKDDTFSVLMSREM
LDGKSEQSELVGVRLNTGGKDYYAIRAEDGKFYDREGSGLTRGFMRFPTMKQFRISSNFN
PRRLNPVTGRVAPHKGVDFAMPVGSPVLAVGDGEVVIAKRSGAAGNYVAIRHGRQYTTRY
MHMHRILVKPGQKVKRGDRIGLSGNTGRSTGPHLHYEFWINQQAVNPLTAKLPRSEGLAG
KERRDYLAQVKEVVPQLKFD