Protein Info for HER17_RS11110 in Pectobacterium carotovorum WPP14
Annotation: asparaginase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 81% identical to ASPG1_ECO57: L-asparaginase 1 (ansA) from Escherichia coli O157:H7
KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 99% identity to pct:PC1_1969)MetaCyc: 81% identical to L-asparaginase 1 (Escherichia coli K-12 substr. MG1655)
Asparaginase. [EC: 3.5.1.1, 3.5.1.38, 3.5.5.4]
Predicted SEED Role
"L-asparaginase I, cytoplasmic (EC 3.5.1.1)" (EC 3.5.1.1)
MetaCyc Pathways
- superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-asparagine degradation I (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- L-asparagine degradation III (mammalian) (2/3 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Cyanoamino acid metabolism
- D-Glutamine and D-glutamate metabolism
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.1
Use Curated BLAST to search for 3.5.1.1 or 3.5.1.38 or 3.5.5.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (339 amino acids)
>HER17_RS11110 asparaginase (Pectobacterium carotovorum WPP14) MRKKSIYVAYTGGTIGMQRSANGYVPVSGHLQQQLAKMPEFHREEMPSFTIHEYDPLIDS SDMTPADWQSIADDIQNHYDDYDGFVILHGTDTMAFTASALSFMLENLAKPVIVTGSQIP LAELRSDGQTNLLNALYVAANHPINEVALFFNNKLLRGNRTTKAHADGFDAFASPNYPPL LEAGIHIRRLAPTVECSNCPPLKVHHITPQPIGVITIYPGISADVISNFLRQPVKALILR SYGVGNAPQSPGLLHELREASARGIVVVNLTQCISGRVNMGGYATGNALAHAGVISGFDM TVEAALTKLHYLLSQQDLSADEIRQLMQQNLHGELSDKD