Protein Info for HER17_RS10515 in Pectobacterium carotovorum WPP14

Annotation: Re/Si-specific NAD(P)(+) transhydrogenase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 33 to 51 (19 residues), see Phobius details amino acids 57 to 75 (19 residues), see Phobius details amino acids 86 to 106 (21 residues), see Phobius details amino acids 125 to 143 (19 residues), see Phobius details amino acids 164 to 181 (18 residues), see Phobius details amino acids 188 to 207 (20 residues), see Phobius details amino acids 216 to 235 (20 residues), see Phobius details amino acids 240 to 260 (21 residues), see Phobius details PF02233: PNTB" amino acids 7 to 460 (454 residues), 670.9 bits, see alignment E=5.2e-206

Best Hits

Swiss-Prot: 89% identical to PNTB_ECO57: NAD(P) transhydrogenase subunit beta (pntB) from Escherichia coli O157:H7

KEGG orthology group: K00325, NAD(P) transhydrogenase subunit beta [EC: 1.6.1.2] (inferred from 99% identity to eca:ECA2205)

MetaCyc: 89% identical to pyridine nucleotide transhydrogenase subunit beta (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-277 [EC: 7.1.1.1]

Predicted SEED Role

"NAD(P) transhydrogenase subunit beta (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2 or 7.1.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (462 amino acids)

>HER17_RS10515 Re/Si-specific NAD(P)(+) transhydrogenase subunit beta (Pectobacterium carotovorum WPP14)
MSGGLVTAAYIVAAILFIFSLAGLSKHETSQQGNIFGISGMALALIATILGPDSGNVGWI
IVAMAIGGSIGIYLARKVEMTEMPELVAILHSFVGLAAVLVGFNSFLDHGEITDPVMVNI
HLTEVFLGIFIGAVTFTGSVIAFGKLRGNISSKPLMLPHRHKMNLAALVVSFLLLLVFVN
TGSVALQVLALILMTAIALVFGWHLVASIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDL
LIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFGTDGVSSGESEEVGEYREASAEDVA
DLLKNSTSVIITPGYGMAVAQAQYPVHDITAKLRARGINVRFGIHPVAGRLPGHMNVLLA
EAKVPYDIVLEMDEINDDFTDTDTVLVIGANDTVNPAAQEDPHSPIAGMPVLEVWKAQNV
IVFKRSMNTGYAGVQNPLFFKENTQMLFGDAKDSVEAILKAL