Protein Info for HER17_RS07385 in Pectobacterium carotovorum WPP14

Annotation: 2-hydroxyacid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 33 to 337 (305 residues), 72.8 bits, E=2.3e-24
• PF02826: 2-Hacid_dh_C
  • amino acids 108 to 310 (203 residues), 184.5 bits, E=1.2e-58

Best Hits

KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 96% identity to pct:PC1_2719)

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
• L-serine biosynthesis I (3/3 steps found)
• L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.95

Protein Sequence (342 amino acids)

>HER17_RS07385 2-hydroxyacid dehydrogenase (Pectobacterium carotovorum WPP14)
MKIVFTAEYGGSLDAFQALGELVVDGWAIGQPKLSEAALIHLAENADVIVTSYDDITARV
IEACPHLRLIACTRANPVNIDIEAARRRGIPVLYTPGRNADAAAELTLALMLNATRHIPQ
AHSALKRGEFTREIHTATQTQDGLRQDVVWDVDKHSPYEVFKGGELRNKTLGLIGYGSIG
HRVGKLARAFGMQLLIADPYVAAEEIDEPGIRKTTLDELFSQSDFVSLHLNSTPQTKGLV
NLDRLRTMRPNAYLINTSRAAVVVEADLITALREKWLAGAALDVYDSEPLWRHHPFITEF
DNVVLTPHIAGATRETLVKHTAMIAADLQRFIRSEPLLYEWK