Protein Info for HER17_RS06495 in Pectobacterium carotovorum WPP14

Annotation: cysteine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 TIGR00435: cysteine--tRNA ligase" amino acids 2 to 460 (459 residues), 649.3 bits, see alignment E=2.1e-199 PF01406: tRNA-synt_1e" amino acids 15 to 313 (299 residues), 490.1 bits, see alignment E=5.5e-151 PF09334: tRNA-synt_1g" amino acids 36 to 105 (70 residues), 25.4 bits, see alignment E=1.2e-09 amino acids 252 to 310 (59 residues), 26.1 bits, see alignment E=7.1e-10 PF09190: DALR_2" amino acids 341 to 402 (62 residues), 95.3 bits, see alignment E=5.1e-31

Best Hits

Swiss-Prot: 97% identical to SYC_PECCP: Cysteine--tRNA ligase (cysS) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 97% identity to pwa:Pecwa_1345)

MetaCyc: 85% identical to cysteine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (461 amino acids)

>HER17_RS06495 cysteine--tRNA ligase (Pectobacterium carotovorum WPP14)
MLKIFNTLSRQKEEFKPIHAGQVGMYVCGITVYDLCHIGHGRTFVAFDVVARYLRYLGYS
LKYVRNVTDIDDKIIKRAIENGETSDQLTTRMIAEMHADFDALNILRPDAEPRATHHIAD
IIEMVETLIARRHAYVASNGDVMFSVDTAPGYGVLSRQDLDQLQAGARVEITEVKRNPMD
FVLWKMSKPGEPHWSSPWGEGRPGWHIECSAMNCKQLGTHFDIHGGGSDLMFPHHENEIA
QSSCAHDGSYVNYWMHSGMVMVDREKMSKSLNNFFTVRDVLAYYDPETVRYFLMSGHYRS
QLNYSEDNLKQARAALDRLYTALRGTDASVAAHGGDEFEARFREAMDDDFNTPEAYSVLF
DMAREVNRLKTEDVQAANQLASALRKLSGVLGLLEQDPEQFLQNGAQVDDEEVKEIEALI
QQRKDARAAKDWALADQARDRLNEMGIVLEDGPQGTIWRRK