Protein Info for HER17_RS06420 in Pectobacterium carotovorum WPP14

Annotation: exopolyphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 511 TIGR03706: exopolyphosphatase" amino acids 16 to 309 (294 residues), 331.8 bits, see alignment E=1.9e-103 PF02541: Ppx-GppA" amino acids 29 to 311 (283 residues), 336.5 bits, see alignment E=1.5e-104 PF21447: Ppx-GppA_III" amino acids 317 to 493 (177 residues), 203.8 bits, see alignment E=1.9e-64

Best Hits

Swiss-Prot: 75% identical to PPX_SALTY: Exopolyphosphatase (ppx) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 98% identity to pct:PC1_2946)

MetaCyc: 75% identical to exopolyphosphatase (Escherichia coli K-12 substr. MG1655)
Exopolyphosphatase. [EC: 3.6.1.11]

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (511 amino acids)

>HER17_RS06420 exopolyphosphatase (Pectobacterium carotovorum WPP14)
MPLTNNEKTEIKPQEFAAIDLGSNSFHMVIARVVNGALQVLGRLKQRVHLADGLDSKNVL
SEEAIQRGLSCLALFAERLQGFPAMNVSIVGTHALRQAVNAQEFLRRAADIIPYPIEIIS
GHEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGEDFEPMLVESRRMGCVSFAQQFF
PNGEISEANFKRARLAAAQKLETLSWEYRIYGWKFALGASGTIKATHEILVEMGEKDGLI
TPERLEMLRTQILQFKHFKALSLPGLSEDRQSVLVPGLAILCGIFDALAIKELRLSDGAL
REGVLYEMEGRFRHQDIRIRTAQSLATHYNIDREQARRVRETTQQLYAQWAEQNPNLVHP
QLEAILNWASMLHEVGLGINHSGMHRHSAYILQNTNLPGFNQEQQLVLSLIVRLHRKAIK
LEELPRLNLFKKKQYLPMMQLLRLATLLNNQRQATTTPESLRLHTDDNYWTLTFPRDFFT
NNTLVQLDLEREQEYWQDVTGWKLIIEEEKA