Protein Info for HER17_RS05935 in Pectobacterium carotovorum WPP14

Annotation: pectinesterase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF01095: Pectinesterase" amino acids 35 to 357 (323 residues), 155.5 bits, see alignment E=6.6e-50

Best Hits

Swiss-Prot: 75% identical to PMEA_DICD3: Pectinesterase A (pemA) from Dickeya dadantii (strain 3937)

KEGG orthology group: K01051, pectinesterase [EC: 3.1.1.11] (inferred from 90% identity to pwa:Pecwa_1228)

MetaCyc: 75% identical to pectin methylesterase A (Dickeya dadantii 3937)
Pectinesterase. [EC: 3.1.1.11]; 3.1.1.11 [EC: 3.1.1.11]

Predicted SEED Role

"Pectinesterase (EC 3.1.1.11)" in subsystem D-Galacturonate and D-Glucuronate Utilization (EC 3.1.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.11

Use Curated BLAST to search for 3.1.1.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (368 amino acids)

>HER17_RS05935 pectinesterase family protein (Pectobacterium carotovorum WPP14)
MIDTSHLCKTLTLAMLIFAPLSLAQAADYNALVSASSGDAKAYKTITEAIASAPADSSPF
VIYVKNGVYHERLTITRPNIHLKGESRNGTVITATTAAGTLKPDGSKWGTYGSNTVKVDA
PDFSARSLTIRNDFDYPANQAKASDDPTKLKDSQAVALLVAENSDRAWFHDVSLVGYQDT
LYVKGGRSFFSQCRISGTVDFIFGDGTALFDNCDIVSRNRTDVKGQPLGYLTAPSTDIKQ
KYGLVITNSRVIKEKGVPAKSYGLGRPWHPTTTFPDGRYANPNAIGQTVFLNTSMDDHIY
GWDKMSGKDKQGATIWFYPQDSRFFEYKSKGAGAEKNDQRRQLSNAEAAEYTADKVLAGW
VPTAPKGK