Protein Info for HER17_RS01510 in Pectobacterium carotovorum WPP14

Annotation: glycerol-3-phosphate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 5 to 323 (319 residues), 204.4 bits, E=5.7e-64
• PF16901: DAO_C
  • amino acids 381 to 479 (99 residues), 51.7 bits, E=1.2e-17

Best Hits

Swiss-Prot: 77% identical to GLPD_ECOLI: Aerobic glycerol-3-phosphate dehydrogenase (glpD) from Escherichia coli (strain K12)

KEGG orthology group: K00111, glycerol-3-phosphate dehydrogenase [EC: 1.1.5.3] (inferred from 98% identity to pwa:Pecwa_4129)

MetaCyc: 77% identical to aerobic glycerol 3-phosphate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate dehydrogenase. [EC: 1.1.5.3]

Predicted SEED Role

"Sn-glycerol-3-phosphate dehydrogenase (Aerobic) (Aerobic glycerol-3-phosphate dehydrogenase)(EC 1.1.99.5)" in subsystem Respiratory dehydrogenases 1 (EC 1.1.99.5)

MetaCyc Pathways

• glycerol and glycerophosphodiester degradation (4/4 steps found)
• sn-glycerol 3-phosphate anaerobic respiration (3/3 steps found)
• glycerol degradation I (3/3 steps found)
• glycerol-3-phosphate shuttle (2/2 steps found)
• glycerol-3-phosphate to fumarate electron transfer (2/2 steps found)
• glycerophosphodiester degradation (2/2 steps found)
• nitrate reduction IX (dissimilatory) (2/2 steps found)
• glycerol 3-phosphate to cytochrome aa₃ oxidase electron transfer (1/2 steps found)
• glycerol-3-phosphate to cytochrome bo oxidase electron transfer (1/2 steps found)
• glycerol-3-phosphate to hydrogen peroxide electron transport (1/2 steps found)

KEGG Metabolic Maps

• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.3

Use Curated BLAST to search for 1.1.5.3 or 1.1.99.5

Protein Sequence (499 amino acids)

>HER17_RS01510 glycerol-3-phosphate dehydrogenase (Pectobacterium carotovorum WPP14)
METKDLIVIGGGINGAGIAADAAGRGLSVLLLEAQDLACATSSASSKLIHGGLRYLEHYE
FRLVSEALSERETLLKMAPHIIFPMRFRLPHQPHLRPAWMIRIGLFMYDNIGKRVSLPAS
KGLKFGADSVLKPELKQGFEYSDCWVDDARLVVLNAQEVTKRGGEVRTRTKVTRARREQG
VWIVDAVDSLTGETFTWRAKGLVNATGPWVKEFFDDGLQLKSPYGIRLIKGSHIVVPKVH
NQPQAYILQNKDHRIVFVIPWQDDYSIIGTTDVEYKGNPHDVKIDDNEVGYLLDVYNDHF
KQQLTRDDIVWTYSGVRPLCDDESDSPQAITRDYTLSVDDDNGQAPLLSVFGGKLTTYRK
LAEHALDKLHKYYPQAGKAWTKGAVLPGGDIAGTRDDYAAALRRRFNLPESLTRRYSRTY
GSNSELILANVKGLSDLGEDFGHDLYEAELRYLVEKEWAVMLDDVIWRRTKLGMRFDDAQ
KQRISDWLANHYQQMAKAG