Protein Info for HER17_RS00975 in Pectobacterium carotovorum WPP14

Annotation: tRNA (uridine(54)-C5)-methyltransferase TrmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 PF05958: tRNA_U5-meth_tr" amino acids 10 to 366 (357 residues), 632.5 bits, see alignment E=1.9e-194 TIGR02143: tRNA (uracil(54)-C(5))-methyltransferase" amino acids 10 to 366 (357 residues), 618.6 bits, see alignment E=1.5e-190

Best Hits

Swiss-Prot: 100% identical to TRMA_PECCP: tRNA/tmRNA (uracil-C(5))-methyltransferase (trmA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00557, tRNA (uracil-5-)-methyltransferase [EC: 2.1.1.35] (inferred from 100% identity to pct:PC1_0191)

MetaCyc: 79% identical to tRNA m5U54 methyltransferase (Escherichia coli K-12 substr. MG1655)
tRNA (uracil-5-)-methyltransferase. [EC: 2.1.1.35]

Predicted SEED Role

No annotation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.35

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (367 amino acids)

>HER17_RS00975 tRNA (uridine(54)-C5)-methyltransferase TrmA (Pectobacterium carotovorum WPP14)
MTPEILPIEQYDDQLAEKTERLRGMMAPFNAPEPVVFRSPVSHYRMRAEFRIWHEGDELY
HIMFDQQTKQRIRVDRFPAASELINRLMPELLAAIQPDSVLRRKLFQIDYLSTLSGEVIV
SLLYHRALDEEWQEHARALRDSLTAQGFRLQLIGRATKTKICLDRDYVDECLPVAGRDMI
YRQVENSFTQPNAAINIQMLEWALDATAGSTGDLLELYCGNGNFSLALARNFERVLATEI
AKPSVAAAQYNIAANQIENVQIIRMAAEEFTQAMQGVRQFNRLQGIDLTSYQCETIFVDP
PRSGLDDETVKLVQAYPRILYISCNPETLSNNLQTLSETHDIRRLALFDQFPYTHHMECG
VLLEKRQ