Protein Info for HER17_RS00915 in Pectobacterium carotovorum WPP14

Annotation: bifunctional aspartate kinase/homoserine dehydrogenase II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 13 to 285 (273 residues), 138.2 bits, E=5.6e-44
• TIGR00657: aspartate kinase
  • amino acids 16 to 454 (439 residues), 214.5 bits, E=1.5e-67
• PF03447: NAD_binding_3
  • amino acids 466 to 601 (136 residues), 68.2 bits, E=1.5e-22
• PF00742: Homoserine_dh
  • amino acids 616 to 804 (189 residues), 140.4 bits, E=8.6e-45

Best Hits

Swiss-Prot: 83% identical to AK2H_ECOLI: Bifunctional aspartokinase/homoserine dehydrogenase 2 (metL) from Escherichia coli (strain K12)

KEGG orthology group: K12525, bifunctional aspartokinase / homoserine dehydrogenase 2 [EC: 1.1.1.3 2.7.2.4] (inferred from 98% identity to eca:ECA4251)

MetaCyc: 83% identical to fused aspartate kinase/homoserine dehydrogenase 2 (Escherichia coli K-12 substr. MG1655)
Aspartate kinase. [EC: 2.7.2.4]; Homoserine dehydrogenase. [EC: 2.7.2.4, 1.1.1.3]

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (8/8 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• norspermidine biosynthesis (4/6 steps found)
• ectoine biosynthesis (3/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• spermidine biosynthesis II (2/4 steps found)
• superpathway of polyamine biosynthesis III (4/8 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.3, 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Protein Sequence (811 amino acids)

>HER17_RS00915 bifunctional aspartate kinase/homoserine dehydrogenase II (Pectobacterium carotovorum WPP14)
MSALGVAPSVTGRQLHKFGGSSLADVKCYLRVAGIMAEYSHPGDLMVVSAAGSTTNQLIS
WLKLSQSDRLSAHQVQQALRRYHSELIAGLLPAQEAEALTAQFIRDLERLAALLDGKITD
AVYAEVVGHGEIWSARLMSAVLNHLDMNAAWLDARDFLCAERAAQPQVDEGRSWPLLQQY
LTQHAGQRLVVTGFISRNDAGETVLLGRNGSDYSATQIGALAGVERVTIWSDVAGVYSAD
PRKVKDACLLPLLRLDEASELARLAAPVLHTRTLQPVSGSDIDLQLRCSYQPEQGSTRIE
RVLASGTGAKIVTSHDDVCLIEVQVPSEHDFALLQKEVEQLLNRAQLKPLAIGVHQDRNL
LQLCYTSEVVDSALQLLAQAALPVELNQRDGLAMVAMVGAGVGKNPLHSHRFYQQLKDQP
IEFVRQADDGISLVAVLRVGPTEHLIRGLHHSLFRAEKRIGLVLFGKGNIGSRWLELFAR
EQSNLSARTGFEFVLAGVVDSTRSLLNYDGLDASRALAFFESEAQERDGEDLFLWMRSHP
FDDLVVLDVTASESVADLYLDFASYGFHVISANKLAGASGGNNYRQIRDAFAKTDRHWLY
NATVGAGLPVNFAVRDLRESGDSILAISGIFSGTLSWLFLQFDGTVPFTELVDQACQQGL
TEPDPRVDLSGQDVMRKLVILAREAGYDIEPSQVRVESLVPPGCEQGSVDYFFENGDSLN
EQMLQRLEAAQEMGLVLRHVARFDSNGKARVGVEAVRPDHPLASLLPGDNVFAIESRWYR
DNPLVIRGPGAGRDVTAGALQSDLNRLAQLL