Protein Info for PS417_05020 in Pseudomonas simiae WCS417

Annotation: fumarylacetoacetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 TIGR01266: fumarylacetoacetase" amino acids 9 to 427 (419 residues), 618.3 bits, see alignment E=3.3e-190 PF09298: FAA_hydrolase_N" amino acids 23 to 120 (98 residues), 71.3 bits, see alignment E=7e-24 PF01557: FAA_hydrolase" amino acids 134 to 424 (291 residues), 127.6 bits, see alignment E=5.6e-41

Best Hits

KEGG orthology group: K01555, fumarylacetoacetase [EC: 3.7.1.2] (inferred from 94% identity to pfs:PFLU1026)

Predicted SEED Role

"Fumarylacetoacetase (EC 3.7.1.2)" in subsystem Homogentisate pathway of aromatic compound degradation or Salicylate and gentisate catabolism (EC 3.7.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1T236 at UniProt or InterPro

Protein Sequence (428 amino acids)

>PS417_05020 fumarylacetoacetase (Pseudomonas simiae WCS417)
MTQPTLTRSWVASANGHTDFPLQNLPLGVFSINGSAPRAGVAIGDSILDLQAAIDEFDGE
ARRAIEATAGGQLNAFFALGRGPRVALRERLLELLAEGSTLHTREAQVLHRAADCQMHVP
AQINDYTDFYVGIEHAQNVGKLFRPDNPLLPNYKYVPIGYHGRASTIRTSGTDVRRPKGQ
TLPAGQSEPTFGPCARLDYELELGIWIGQGNEMGDSIAIGDAADHIAGFCLLNDWSARDI
QAWEYQPLGPFLSKSFITTISPWVVTAEALEPFRKAQPARPEGDPQPLSYLLDKRDQAAG
ALDIELEVLLTTAAMREQNLPAHRLALSNSLHMYWTVAQLVAHHSVNGCQLQAGDLFGSG
TLSGPQAGQFGSLLEMTEGGKKPVELPSGEVRKFLEDGDEIILRARCNREGFASIGFGEC
RGTVIAAR