Protein Info for PS417_05015 in Pseudomonas simiae WCS417

Annotation: homogentisate 1,2-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 TIGR01015: homogentisate 1,2-dioxygenase" amino acids 3 to 421 (419 residues), 636.6 bits, see alignment E=9e-196 PF20510: HgmA_N" amino acids 4 to 269 (266 residues), 394.8 bits, see alignment E=1.9e-122 PF04209: HgmA_C" amino acids 270 to 421 (152 residues), 263.9 bits, see alignment E=3.9e-83

Best Hits

Swiss-Prot: 89% identical to HGD_PSEPF: Homogentisate 1,2-dioxygenase (hmgA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K00451, homogentisate 1,2-dioxygenase [EC: 1.13.11.5] (inferred from 96% identity to pfs:PFLU1025)

Predicted SEED Role

"Homogentisate 1,2-dioxygenase (EC 1.13.11.5)" in subsystem Homogentisate pathway of aromatic compound degradation (EC 1.13.11.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1TIB6 at UniProt or InterPro

Protein Sequence (427 amino acids)

>PS417_05015 homogentisate 1,2-dioxygenase (Pseudomonas simiae WCS417)
MNLEYLSGFGNEFASEALPGALPIGQNSPQKAPYGLYTELFSGTAFTMVRSEARRTWLYR
IQPSANHPAFIKLERQLAGGPLGAVTPNRLRWNPLDMPSEPTDFIDGLVGMVANSGAEKP
SGISIYTYRANRSMERVFFNADGELLIVPELGRLRIATELGVLEVEPLEIVVLPRGLKFR
VELLDTQARGYVAENHGAPLRLPDLGPIGSNGLANPRDFLTPVARYEDVKKPTTLVQKFL
GELWACELDHSPLNVVAWHGNNVPYKYDLRRFNTLGTVSFDHPDPSIFTVLTSPTSTHGL
ANLDFVIFPPRWMVAENTFRPPWFHRNLMNEYMGLIQGAYDAKAEGFLPGGASLHSCMSA
HGPDGETCTKAINVDLAPHKIDNTMAFMFETSQVLRPTQFALDCPQLQNTYDACWASLPA
TFNPNRR