Protein Info for GFF98 in Xanthobacter sp. DMC5

Annotation: putative lipid II flippase MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 31 to 51 (21 residues), see Phobius details amino acids 86 to 108 (23 residues), see Phobius details amino acids 128 to 153 (26 residues), see Phobius details amino acids 159 to 184 (26 residues), see Phobius details amino acids 191 to 214 (24 residues), see Phobius details amino acids 235 to 248 (14 residues), see Phobius details amino acids 255 to 275 (21 residues), see Phobius details amino acids 281 to 299 (19 residues), see Phobius details amino acids 311 to 332 (22 residues), see Phobius details amino acids 352 to 369 (18 residues), see Phobius details amino acids 389 to 406 (18 residues), see Phobius details amino acids 412 to 435 (24 residues), see Phobius details amino acids 445 to 467 (23 residues), see Phobius details amino acids 479 to 500 (22 residues), see Phobius details PF03023: MurJ" amino acids 29 to 450 (422 residues), 178.6 bits, see alignment E=9.3e-57

Best Hits

KEGG orthology group: K03980, virulence factor (inferred from 56% identity to azc:AZC_4303)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (512 amino acids)

>GFF98 putative lipid II flippase MurJ (Xanthobacter sp. DMC5)
MSLLARTSIVSAATLVSRVLGFARDAATAAVLGVGPVADALAAAMALPLLARRLLAEGAF
NLAFIPALARTSGAAGSRDEEAGRLAGATLLLLCGLLLVFAAVVAVFMPQVIGLMAPGFE
AGGQRADVAILCGRVVVLYIPLAGLAAIFGGIANAAHRVLLPALAPAAANVTVLAVIGVL
LAKGLMESDTAAFAIAAAQVGAGCAQFGIMRLAARGCPAQPLFRVLTVRDLRAALGVLRA
ASPALLFAGLSQLRFIIVVAAVSATPGAAAALNYAQRLMDLPLGLVGASAGAVLVPALLR
RGRAEAADDAGRAVLAALAFALPAAVGLAVLAEPIVVTLFQRGSFDSEDARFTAALLGVL
SLSLPAQGLERILSAAASTSGQIKAAERIALGSLLLCMVAAAGLGTVGGPLIAAGAVALS
AVASILALGGVLIASGALRFSAPVLLSAAGLAAASLAMGGGVGVLAATWPVPGGTLAGIL
RLVGLVASGGVIYGAGALVVKAGVSKAWTGRV