Protein Info for GFF978 in Sphingobium sp. HT1-2

Annotation: Gamma-glutamyltranspeptidase (EC 2.3.2.2) @ Glutathione hydrolase (EC 3.4.19.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 569 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR00066: gamma-glutamyltransferase" amino acids 37 to 561 (525 residues), 402.6 bits, see alignment E=1.2e-124 PF01019: G_glu_transpept" amino acids 49 to 564 (516 residues), 487.2 bits, see alignment E=3.4e-150

Best Hits

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 82% identity to sch:Sphch_2035)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.2.2

Use Curated BLAST to search for 2.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (569 amino acids)

>GFF978 Gamma-glutamyltranspeptidase (EC 2.3.2.2) @ Glutathione hydrolase (EC 3.4.19.13) (Sphingobium sp. HT1-2)
MTSRLTGLLAPFALIAPLALAPATALAREPVALNATVSAADPRAAAAGQEILRKGGSATD
AAIAMMLALNVVEPHNSGIGGGGFLMHHDGASGVLESIDGRETAPAAARPDRFMGADGKP
LPFVQAWPGGYSVGVPGNLRMAWEAHKKWGKLPWADLFQPAIRLADDGFQLGERTATALK
ALQNIWADFPEIRSYYWIDGQLPAVGTTLKNPPLAALFKRIAAEGPDAFYLGENAQAIAK
AVTNAPRNPVPMTTDDLANYQVKDRKPVCGHYRAYTVCGMGPASAGGITVLQVLGMVERF
PLAQWGKDDPRSWHVIGEAMQLAYADRDTWLGDPEFVHVPIAGLIDPAYLKQRSGEIRLN
KALTAYKPGLPAGAQPRTSALPQPESGTSHFVAVDRNGDIAAWTSTIESFFGSQLVANGV
VLNNELTDFSFTPEKNGAPVANRVEPGKRPLSSMSPTIVYDAKGMPIFTVGAAGGKTIIM
QVAKALIAHFDWGLSAQDSIALGLEFFNGDGLVLEQGTSLEAMKAPLEKLGHRVTISRLG
LKANAAERTADGHWIGAADPRSPGVSLQE