Protein Info for GFF976 in Xanthobacter sp. DMC5

Annotation: Glycerate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 17 to 328 (312 residues), 101 bits, E=6.4e-33
• PF02826: 2-Hacid_dh_C
  • amino acids 113 to 297 (185 residues), 184.4 bits, E=1.9e-58
• PF03446: NAD_binding_2
  • amino acids 155 to 261 (107 residues), 21.8 bits, E=2.5e-08

Best Hits

Swiss-Prot: 44% identical to GYAR_THELN: Glyoxylate reductase (gyaR) from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C)

KEGG orthology group: K00015, glyoxylate reductase [EC: 1.1.1.26] (inferred from 95% identity to xau:Xaut_4126)

MetaCyc: 37% identical to hydroxypyruvate reductase subunit (Hyphomicrobium methylovorum GM2)
Hydroxypyruvate reductase. [EC: 1.1.1.81]

Predicted SEED Role

"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81)" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)

MetaCyc Pathways

• photorespiration I (7/9 steps found)
• photorespiration III (7/9 steps found)
• photorespiration II (7/10 steps found)
• L-serine biosynthesis II (1/4 steps found)
• D-xylose degradation IV (3/7 steps found)
• formaldehyde assimilation I (serine pathway) (7/13 steps found)
• L-arabinose degradation IV (3/8 steps found)
• superpathway of pentose and pentitol degradation (9/42 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.79, 1.1.1.81

Use Curated BLAST to search for 1.1.1.26 or 1.1.1.79 or 1.1.1.81

Protein Sequence (333 amino acids)

>GFF976 Glycerate dehydrogenase (Xanthobacter sp. DMC5)
MVRRKPLVVVTRKLPDSVETRMRELFDAKLNIDDVPMDQAALVEAVKTADVLVPTVTDRI
DTAVLSQAGENLRLIASFSNGVDHIDVASALNRGITVTNTPGVLTEDTADMTMALILSVP
RRLAEGAQVMIADKEEWAGWSPTWMLGRRIWGKRLGIIGMGRIGQAVARRAKAFGLQIHY
HNRRRLNSGIEEMLDATYWDSLDQMLARMDIVSVNCPHTPATYHLLSARRLKLLKPDAYV
VNTARGEVIDENALTRLLEAGEIAGAGLDVFEHEPAVNPKLVRLAKQGKVVLLPHLGSAT
LEGRVDMGEKVIVNIKTFMDGHRPPDRVLPAML