Protein Info for GFF974 in Sphingobium sp. HT1-2

Annotation: Iron-sulfur cluster assembly protein SufB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 TIGR01980: FeS assembly protein SufB" amino acids 23 to 485 (463 residues), 632.2 bits, see alignment E=2.2e-194 PF19295: SufBD_N" amino acids 154 to 215 (62 residues), 38.8 bits, see alignment E=7.6e-14 PF01458: SUFBD_core" amino acids 223 to 465 (243 residues), 270.2 bits, see alignment E=1.4e-84

Best Hits

Swiss-Prot: 68% identical to Y074_SYNY3: UPF0051 protein slr0074 (slr0074) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K09014, Fe-S cluster assembly protein SufB (inferred from 95% identity to sjp:SJA_C1-33990)

Predicted SEED Role

"Iron-sulfur cluster assembly protein SufB" in subsystem Staphylococcal phi-Mu50B-like prophages

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>GFF974 Iron-sulfur cluster assembly protein SufB (Sphingobium sp. HT1-2)
MTEEVSTVRNQEALEAAERASTYEHGWSSAIEQDFAPKGLNEDTVRFISAKKNEPQWLLD
WRLKAFAMWQKMEAPDWAKLNVPPIDYQDAYYYAEPKKKVELDSLDQVDPEILATYQKLG
IPIAEQEMLAGVKGSRKIAVDAVFDSVSVATTFRKELEEAGVIFRSISEAVREFPDLVKK
WLGKVVPMHDNYFATLNCAVFSDGTFVYIPKGVRCPMELSTYFRINAENTGQFERTLIVA
DDGAYVSYLEGCTAPMRDENQLHAAVVELVALDDAEIKYSTVQNWYPGDENGKGGIYNFV
TKRALCQGRNSKVSWTQVETGSAITWKYPSCVLNGENSVGEFYSVALTNNLQQADTGTKM
IHNGKGSRSTIVSKGISAGRSNNTYRGLVRVAPGAEGVRNFTQCDSLLLGDQCGAHTVPY
IEVRNPSAQIEHEATTSKISDDQLFYAMQRGLDQESAVSLIVNGFAKEVLQQLPMEFAVE
AQKLLGISLEGSVG