Protein Info for GFF970 in Sphingobium sp. HT1-2

Annotation: Cysteine desulfurase (EC 2.8.1.7) => SufS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01979: cysteine desulfurase, SufS family
  • amino acids 19 to 401 (383 residues), 550.7 bits, E=9.2e-170
• PF00266: Aminotran_5
  • amino acids 21 to 389 (369 residues), 468.5 bits, E=2.4e-144
• PF00155: Aminotran_1_2
  • amino acids 65 to 190 (126 residues), 33.3 bits, E=4.6e-12
• PF01053: Cys_Met_Meta_PP
  • amino acids 104 to 222 (119 residues), 28.9 bits, E=6.8e-11

Best Hits

Swiss-Prot: 50% identical to SUFS_DICD3: Cysteine desulfurase (sufS) from Dickeya dadantii (strain 3937)

KEGG orthology group: K11717, cysteine desulfurase / selenocysteine lyase [EC: 2.8.1.7 4.4.1.16] (inferred from 93% identity to sjp:SJA_C1-33940)

MetaCyc: 48% identical to L-cysteine desulfurase (Escherichia coli K-12 substr. MG1655)
Selenocysteine lyase. [EC: 4.4.1.16]; Cysteine desulfurase. [EC: 4.4.1.16, 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), SufS subfamily" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of L-alanine biosynthesis (4/4 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• superpathway of thiamine diphosphate biosynthesis II (7/11 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• superpathway of thiamine diphosphate biosynthesis I (6/10 steps found)
• molybdopterin biosynthesis (3/6 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (3/7 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (2/6 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (2/10 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)

KEGG Metabolic Maps

• Selenoamino acid metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7 or 4.4.1.16

Protein Sequence (401 amino acids)

>GFF970 Cysteine desulfurase (EC 2.8.1.7) => SufS (Sphingobium sp. HT1-2)
MTDLAQGLRLRDDFPGVGDWHYLDSAATAQKPTAVIDAIARAYGPDYATVHRGVYERSAN
MTLAYEAARRKVAGFIGAASDSEIVYVRGATEGINLVAQCWAGTQLKAGDRILLSTLEHH
SNIVPWQIVAEKVGAQIDVVPLTQDGRIDLDAMRAMITPQHRMVALAHVSNVLGSVLDCR
RAADIAHMVGAKILIDGCQAVPRLAVDVQALDCDFYVFSAHKLYGPTGIGVLWGRKELLD
AMPPYQGGGSMIDKVTFEKTTYAPAPTRFEAGTPHIVGVVGLSAAIDYVQGIGLDAIHAH
ECAMVAKARTAISQINSVRVFGPEDSAGILSFEVEGVHPHDVGTILDETGVAIRAGHHCA
QPLMRHLGVEATARASFGIYSDDSDVDALVKGIERVRKIFG