Protein Info for GFF97 in Xanthobacter sp. DMC5

Annotation: ATP-dependent zinc metalloprotease FtsH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 104 to 125 (22 residues), see Phobius details PF06480: FtsH_ext" amino acids 9 to 97 (89 residues), 62 bits, see alignment E=1.7e-20 TIGR01241: ATP-dependent metallopeptidase HflB" amino acids 105 to 598 (494 residues), 789.3 bits, see alignment E=7.4e-242 PF06068: TIP49" amino acids 183 to 226 (44 residues), 24.2 bits, see alignment 5.4e-09 PF07728: AAA_5" amino acids 192 to 314 (123 residues), 30.3 bits, see alignment E=1.2e-10 PF00004: AAA" amino acids 193 to 325 (133 residues), 162.6 bits, see alignment E=2e-51 PF17862: AAA_lid_3" amino acids 348 to 391 (44 residues), 63.5 bits, see alignment 3e-21 PF01434: Peptidase_M41" amino acids 407 to 596 (190 residues), 237.3 bits, see alignment E=3.8e-74

Best Hits

Swiss-Prot: 69% identical to FTSH_BARBK: ATP-dependent zinc metalloprotease FtsH (ftsH) from Bartonella bacilliformis (strain ATCC 35685 / NCTC 12138 / KC583)

KEGG orthology group: K03798, cell division protease FtsH [EC: 3.4.24.-] (inferred from 96% identity to xau:Xaut_3083)

MetaCyc: 60% identical to ATP-dependent zinc metalloprotease FtsH (Escherichia coli K-12 substr. MG1655)
3.4.24.-

Predicted SEED Role

"Cell division protein FtsH (EC 3.4.24.-)" in subsystem Bacterial Cell Division (EC 3.4.24.-)

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (640 amino acids)

>GFF97 ATP-dependent zinc metalloprotease FtsH (Xanthobacter sp. DMC5)
MNANLRNFALWVIIVLLLLALFSLFNSPGQRTNANEISFSQLLNDVDAGKVREVVIEGPN
ITGTYSDRSGSFQTYAPNDPSLVQRLYGKGVQITARAPSDNVPWFVSLLLSWLPFLALIG
VWIFLSRQMQGAGGKAMGFGKSRAKLLTEAHGRVTFDDVAGIDEAKSDLTEIVEFLRDPQ
KFQRLGGRIPRGVLLVGPPGTGKTLLARAIAGEANVPFFTISGSDFVEMFVGVGASRVRD
MFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLLVEMDGFEANEGIILIA
ATNRPDVLDPALLRPGRFDRQVIVPNPDVVGREQILKVHARKIPVAPDVNLKTIARGTPG
FSGADLANLCNEAALMAARRNKRMVTMAEFEDAKDKVMMGAERRSLVMTEEEKMLTAYHE
GGHAIVALNVPATDPVHKATIIPRGRALGMVMQLPERDKLSMSYEQMTSRLAIMMGGRVA
EELVFGHDKVTSGAASDIEQATRLAKMMVTRWGFSDLLGQVAYGENNEDVFLGMSMQRHQ
NVSEATAQTIDKEVRRLVDEGYTEAKRILTEKYDDLEALARGLLEYETLSGDEIVDLLDG
KTPNRESVLEPSNPRGSAIPTTKRPRPGGAGGSIEPQPQA