Protein Info for GFF964 in Xanthobacter sp. DMC5

Annotation: Riboflavin biosynthesis protein RibBA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13443: HTH_26
  • amino acids 3 to 62 (60 residues), 22.7 bits, E=2e-08
• TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • amino acids 79 to 274 (196 residues), 234.9 bits, E=3.1e-74
• PF00926: DHBP_synthase
  • amino acids 83 to 273 (191 residues), 272.6 bits, E=3e-85
• PF00925: GTP_cyclohydro2
  • amino acids 283 to 431 (149 residues), 107.9 bits, E=8.3e-35

Best Hits

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 89% identity to xau:Xaut_4136)

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25)" in subsystem Molybdenum cofactor biosynthesis or Riboflavin, FMN and FAD metabolism (EC 3.5.4.25, EC 4.1.99.12)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• toxoflavin biosynthesis (3/7 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25, 4.1.99.12

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Protein Sequence (436 amino acids)

>GFF964 Riboflavin biosynthesis protein RibBA (Xanthobacter sp. DMC5)
MKLAEWLSTTGTKRSAFARQVGMSPASVTALCNDERAWISRETAEKIAEATGQQVTPNDF
LGLSTPLTRGLPVTDTTQARVQSAIEAIARGEIIVVTDDDDRENEGDLILAASLATPEKM
AFVIRNTSGIVCTPLPPSEAKRLRLDPMVSNNDAPMGTAFTVSIDVRHGTTTGISAEERT
NTVRALANPNMGAGDFVRPGHVFPLIAREGGVLIRSGHTEAAVDLCRLAGLPPIGVIAEL
VNDDGTVQRGPQVTAFAEKHNLTRISVAEIIAYRQAREKLVTRAAEFPVPTIVGELQGYS
YVTPFDPVNHLALVKGRIGDGEKVLVRLHRADAIADAFSGGKVLQKSLERIEAEGRGVLV
YLRDGTSGVPAVAMGQNSEKSTSELERDRHWREVGLGAQILRDLGIQSIRLLSSKTRTYV
GLAGFGIEILGTETLD