Protein Info for GFF955 in Sphingobium sp. HT1-2

Annotation: Ribosomal RNA adenine dimethylase domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 215 PF05175: MTS" amino acids 46 to 188 (143 residues), 23.3 bits, see alignment E=6.7e-09 PF13649: Methyltransf_25" amino acids 67 to 166 (100 residues), 29 bits, see alignment E=2.2e-10 PF08241: Methyltransf_11" amino acids 68 to 170 (103 residues), 22.7 bits, see alignment E=1.9e-08

Best Hits

KEGG orthology group: None (inferred from 71% identity to sch:Sphch_2013)

Predicted SEED Role

"phospholipid N-methyltransferase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (215 amino acids)

>GFF955 Ribosomal RNA adenine dimethylase domain protein (Sphingobium sp. HT1-2)
MASLPFKDKKTRISTRGGSRSFRSQWAMFFRQFMKHPGMIGSVIPSSSQLVARSLDGVDW
ARTRLFVEYGPGVGTFTQAILDRMHPDAILLAIDLNLDFVAYLEDAIDDPRLRVVHGSAA
DVRRFVKEAGYQKADYVLSGLPFSTLPAGVGETICEETRAVLRPGGSFIIYQYSRYVRRL
IDPLFGQVSDELEWRNIPPCRLFRADKDEALAQAA