Protein Info for PS417_04840 in Pseudomonas simiae WCS417

Annotation: glycosyl transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 45 to 73 (29 residues), see Phobius details amino acids 364 to 391 (28 residues), see Phobius details amino acids 397 to 417 (21 residues), see Phobius details amino acids 429 to 447 (19 residues), see Phobius details amino acids 475 to 495 (21 residues), see Phobius details PF13641: Glyco_tranf_2_3" amino acids 90 to 345 (256 residues), 146.1 bits, see alignment E=1.8e-46 PF13632: Glyco_trans_2_3" amino acids 186 to 411 (226 residues), 97.8 bits, see alignment E=7.8e-32

Best Hits

Swiss-Prot: 89% identical to ALG8_PSESM: Glycosyltransferase alg8 (alg8) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: None (inferred from 99% identity to pfs:PFLU0989)

MetaCyc: 82% identical to mannuronosyl transferase catalytic subunit (Pseudomonas aeruginosa)
Alginate synthase. [EC: 2.4.1.33]

Predicted SEED Role

"Alginate biosynthesis protein Alg8" in subsystem Alginate metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U515 at UniProt or InterPro

Protein Sequence (496 amino acids)

>PS417_04840 glycosyl transferase (Pseudomonas simiae WCS417)
MTLMSKLKHVLLQSAGWLFFLSLLMGLALLLPASTFDSESKNFIFLIGAVGIWRYSMGAT
HFFRGMLFLYVVYPHLRRKVRKLGKAADPSHVFLMVTSFRIDALTTAQVYSSVIREAIEC
GFPTTVVCSLVEMSDELLVKSLWERMNPPEHVKLDFVRIAGTGKRDGLAFGFRAISRHLP
DDRAVVAVIDGDTVLAEGVVRKTVPWFQLFDNVGGLTTNEFCEVRGGYIMSEWHKLRFAQ
RHINMCSMALSKRVLTMTGRMSVFRASVVTDPGFIADVESDSLQHWRLGRFKFLTGDDKS
SWFSLMRLGYDTFYVPDAAIHTVEHPPEKSFIKASRKLMFRWYGNNLRQNSRALGLGMRR
LGLFTSIVLFDQRVSMWTSLLGLTVAIIATFKYGGAFILAYLLWIGITRLILTLLLSCSG
HKIGPAYPVILYYNQIMGALVKIYVFFRLDQQSWTRQDTKLTRDLASFQRWFNTWSSRTM
TFSAGSIFVAVLLMMV