Protein Info for GFF952 in Methylophilus sp. DMC18

Annotation: NADH-quinone oxidoreductase subunit M

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01972: proton-translocating NADH-quinone oxidoreductase, chain M
  • amino acids 12 to 491 (480 residues), 613.1 bits, E=1.7e-188
• PF00361: Proton_antipo_M
  • amino acids 138 to 421 (284 residues), 261.7 bits, E=4.1e-82

Best Hits

Swiss-Prot: 44% identical to NUOM_RICPR: NADH-quinone oxidoreductase subunit M (nuoM) from Rickettsia prowazekii (strain Madrid E)

KEGG orthology group: K00342, NADH dehydrogenase I subunit M [EC: 1.6.5.3] (inferred from 77% identity to mfa:Mfla_2049)

MetaCyc: 39% identical to NADH:quinone oxidoreductase subunit M (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• aerobic respiration III (alternative oxidase pathway) (3/3 steps found)
• NADH to cytochrome bo oxidase electron transfer I (2/2 steps found)
• aerobic respiration I (cytochrome c) (3/4 steps found)
• NADH to cytochrome bd oxidase electron transfer I (1/2 steps found)
• NADH to dimethyl sulfoxide electron transfer (1/2 steps found)
• NADH to fumarate electron transfer (1/2 steps found)
• NADH to hydrogen peroxide electron transfer (1/2 steps found)
• NADH to trimethylamine N-oxide electron transfer (1/2 steps found)
• nitrate reduction VIII (dissimilatory) (1/2 steps found)
• Fe(II) oxidation (2/6 steps found)
• NAD(P)/NADPH interconversion (2/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Protein Sequence (502 amino acids)

>GFF952 NADH-quinone oxidoreductase subunit M (Methylophilus sp. DMC18)
MMQADLTSISWLSLAVWLPVLSGVLVLLLGGDQKATLTRWLALVGSLVSFLVTVPLYTQF
DIHDGFFQFEELLPWVPAFNMHYHLGVDGFSMPLVLLTSFTTVIVVLAGWEVITKHVAQY
MAAFLIMSGIMIGVFTALDAILYYVFWEAMLIPMFLVIGIWGGPNRVYATIKFFLYTLLG
SLLMLVAFIYLYHQTGSFELADYYLLPLSLQAQIYIFIAFFMAFAVKIPMWPVHTWLPDA
HVEAPTGGSVVLAAIALKLGGYSFLRFAMPIAPDAAHYFTSAMVALSLIAVVYIALVALV
QKDMKKLIAYSSISHMGFVTLGFFMFSQLALEGALVQMISHGFISSAMFLSVGVLYDRVH
SREISAYGGVVNKMPVFAAFAVLFAMANSGLPGTSGFVGEFMVILAAVKFNFWVAFFAAT
TLIFGAAYTLWMTKRVFFGNVTNHHVAELSDLSKREFLILGILAILVIGFGVYPQALTEV
MQATSTEFLKHMAISKLPVTGY