Protein Info for PS417_04820 in Pseudomonas simiae WCS417

Annotation: poly(beta-D-mannuronate) C5 epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF13229: Beta_helix" amino acids 268 to 337 (70 residues), 26.6 bits, see alignment E=4.4e-10 amino acids 333 to 425 (93 residues), 36.2 bits, see alignment E=4.8e-13 PF05048: NosD" amino acids 274 to 377 (104 residues), 36.2 bits, see alignment E=4.6e-13 amino acids 363 to 429 (67 residues), 32.5 bits, see alignment E=5.9e-12 TIGR03804: parallel beta-helix repeat" amino acids 381 to 419 (39 residues), 23.9 bits, see alignment 1.2e-09

Best Hits

Swiss-Prot: 98% identical to ALGG_PSEFL: Mannuronan C5-epimerase (algG) from Pseudomonas fluorescens

KEGG orthology group: None (inferred from 99% identity to pfs:PFLU0985)

MetaCyc: 68% identical to mannuronan C5 epimerase (Pseudomonas aeruginosa)
RXN-9839 [EC: 5.1.3.37]; TRANS-RXN-269 [EC: 5.1.3.37]

Predicted SEED Role

"Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-)" in subsystem Alginate metabolism (EC 5.1.3.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.1.3.- or 5.1.3.37

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TV03 at UniProt or InterPro

Protein Sequence (529 amino acids)

>PS417_04820 poly(beta-D-mannuronate) C5 epimerase (Pseudomonas simiae WCS417)
MGACAMNPQALKGSAMLAAAMLLASAAAMADVAPQAKAPTIAKELQQAKTYTISSPPTAP
LEMAKPALPDLSGYTDAAMEKKIVRAKPGKISIRRMMQEDALKDFIGGDNKMAEWVVRQH
GIPQAIFVDDGYMNLKDLLGKVPKQYLSETSPGVFLAKLPIVVGRKGILEIDKKTQELRL
SQEGGSFLINDGQLFVRDTKVTGWSEKANGPALFKSPKEFRPFLLAWGGTETYISNTKMA
SFGYANSKSYGVSISQYTPNMAKVLKRPEPTGWIINSEFSDMWYGFYCYETTNFVIKGNT
YKDNIVYGIDPHDRSHGLIIADNTVYGTKKKHGIIISREVNDSFIFNNRSYDNKLSGLVL
DRNSVNNIVADNEFYRNHTDGITLYESGDNLLWGNKVIANRRHGIRVRNSVNIKLYENTS
MANGLTGLYGHIKDLTDTDRDIALDPFDAKVSLIVVGGELAGNGSGPLSIDSPLSVELYR
VSMLAPTKSSGISFNGVLGDRQEEILDLLVRQQKAVLIDPVERQTELQD