Protein Info for GFF949 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868
Annotation: Cytosol aminopeptidase PepA (EC 3.4.11.1)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to AMPA_SALPK: Probable cytosol aminopeptidase (pepA) from Salmonella paratyphi A (strain AKU_12601)
KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to sei:SPC_4610)MetaCyc: 98% identical to aminopeptidase A/I (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Leucyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.1, 3.4.11.9]
Predicted SEED Role
"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)
MetaCyc Pathways
- muropeptide degradation (4/4 steps found)
- γ-glutamyl cycle (5/6 steps found)
- glutathione degradation (DUG pathway) (2/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.4.11.9
Use Curated BLAST to search for 3.4.11.1 or 3.4.11.9 or 3.4.13.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (503 amino acids)
>GFF949 Cytosol aminopeptidase PepA (EC 3.4.11.1) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868) MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYISALLRRGELEGKPGQTL LLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLNDTGSMEAVCFLTELHVKGR NNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFNVPTRRELTSGERAIQHGLAI AAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNVITRVIGEQQMRELGMNAYLAVG HGSQNESLMSVIEYKGNPSEDARPIVLVGKGLTFDSGGISIKPSEGMDEMKYDMCGAAAV YGVMRMVAELQLPINVIGVLAGCENMPGGRAYRPGDVLTTMSGQTVEVLNTDAEGRLVLC DVLTYVERFEPEAVIDVATLTGACVIALGHHITGLMSNHNPLAHELIGASEQAGDRAWRL PLGDEFQEQLESNFADMANIGGRPGGAITAGCFLSRFTRKYNWAHLDIAGTAWRSGKAKG ATGRPVALLSQFLLNRAGFNGEE