Protein Info for GFF948 in Xanthobacter sp. DMC5

Annotation: Triosephosphate isomerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00121: TIM
  • amino acids 7 to 247 (241 residues), 289.6 bits, E=1e-90
• TIGR00419: triose-phosphate isomerase
  • amino acids 8 to 238 (231 residues), 170.8 bits, E=2e-54

Best Hits

Swiss-Prot: 99% identical to TPIS_XANFL: Triosephosphate isomerase (tpiA) from Xanthobacter flavus

KEGG orthology group: K01803, triosephosphate isomerase (TIM) [EC: 5.3.1.1] (inferred from 68% identity to xau:Xaut_4380)

MetaCyc: 42% identical to triosephosphate isomerase subunit (Lactobacillus delbrueckii bulgaricus)
Triose-phosphate isomerase. [EC: 5.3.1.1]

Predicted SEED Role

"Triosephosphate isomerase (EC 5.3.1.1)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or MLST (EC 5.3.1.1)

MetaCyc Pathways

• Calvin-Benson-Bassham cycle (12/13 steps found)
• gluconeogenesis I (12/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• ethene biosynthesis V (engineered) (20/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• homolactic fermentation (10/12 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• glycolysis III (from glucose) (9/11 steps found)
• oxygenic photosynthesis (13/17 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (13/17 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycolysis II (from fructose 6-phosphate) (8/11 steps found)
• glycolysis IV (7/10 steps found)
• glycolysis I (from glucose 6-phosphate) (9/13 steps found)
• glycerol degradation to butanol (11/16 steps found)
• gluconeogenesis III (8/12 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• glycolysis V (Pyrococcus) (6/10 steps found)
• sucrose degradation V (sucrose α-glucosidase) (2/5 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (10/18 steps found)
• superpathway of hexitol degradation (bacteria) (9/18 steps found)
• superpathway of N-acetylneuraminate degradation (11/22 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (25/56 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Fructose and mannose metabolism
• Glycolysis / Gluconeogenesis
• Inositol phosphate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.1

Protein Sequence (249 amino acids)

>GFF948 Triosephosphate isomerase (Xanthobacter sp. DMC5)
MTAERRPLIAGNWKMNGLRAAKSELRTVAAGLAGLSAETMICPPFTLIGAFAADVAGTQL
MIGGQDCHPAESGAHTGDISAEMLRDAGAVAVILGHSERRIDHQEGDAIVKAKVKAAWRA
GLLPVVCVGETLVERDAGEAAAVVTRQVRQSVPEGATAVNLVVAYEPIWAIGTGRTPTTD
DVAEVHGAIRHILGERFGAEANGIRILYGGSVKPDNAAALLATANVDGALVGGASLKAAD
FLAIARAVG