Protein Info for PS417_04810 in Pseudomonas simiae WCS417

Annotation: poly(beta-D-mannuronate) lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF05426: Alginate_lyase" amino acids 59 to 300 (242 residues), 223.1 bits, see alignment E=2.4e-70

Best Hits

Swiss-Prot: 97% identical to ALGL_PSEFL: Alginate lyase (algL) from Pseudomonas fluorescens

KEGG orthology group: K01729, poly(beta-D-mannuronate) lyase [EC: 4.2.2.3] (inferred from 97% identity to pfs:PFLU0983)

Predicted SEED Role

"Alginate lyase precursor (EC 4.2.2.3)" in subsystem Alginate metabolism (EC 4.2.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.2.3

Use Curated BLAST to search for 4.2.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UAI6 at UniProt or InterPro

Protein Sequence (369 amino acids)

>PS417_04810 poly(beta-D-mannuronate) lyase (Pseudomonas simiae WCS417)
MQKLLIPTLLGLAMFAGSVNAAAPLRPPQGYFAPIEAFKTGDFKNDCDAMPAPYTGSLQF
RSKYEGSDKARSTLNVQSEKAFRDSTADITKLEKDTSKRVMQFMRDGRPEQLECTLNWLA
TWARADALMSKDFNHTGKSMRKWALGSMASAYVRLKFSESHPLANHQQESQLIEAWFNKL
ADQVVSDWDNLPLEKTNNHSYWAAWSVMATSVATNRRDLFDWAVKEYKVGANQVDDQGFL
PNELKRQQRALSYHNYALPPLSMIASFALVNGVDLRQENNSALKRLGEKVLAGVKDPEIF
EQKNGKEQDMKDLKEDMKYAWLEPFCTLYTCAPDVIERKHGMQPFKTFRLGGDLTKVYDP
AHEKGNKGS