Protein Info for PS417_04700 in Pseudomonas simiae WCS417

Annotation: RND transporter MFP subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 388 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 40 to 371 (332 residues), 246.9 bits, see alignment E=1.2e-77 PF13533: Biotin_lipoyl_2" amino acids 65 to 112 (48 residues), 55.8 bits, see alignment 4.7e-19 PF16576: HlyD_D23" amino acids 66 to 291 (226 residues), 47.9 bits, see alignment E=1.6e-16 PF13437: HlyD_3" amino acids 175 to 282 (108 residues), 24.5 bits, see alignment E=6e-09

Best Hits

KEGG orthology group: None (inferred from 96% identity to pfs:PFLU0961)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See U1T2A0 at UniProt or InterPro

Protein Sequence (388 amino acids)

>PS417_04700 RND transporter MFP subunit (Pseudomonas simiae WCS417)
MQIQRKTALIAGVLVVLAVAAWALTRPAKSKLAAPTAIPVRVVSVAQQDIPRFVSGIGSV
LSLHSVVIRPQVDGILTKLLVKEGQLVKAGDLLATIDDRSIRASLDQAKAQLGESQAQLQ
VALVNLKRYKALSVDDGVSKQTYDQQQALVNQLKATAQGNQAAIDSAQVQLSYTQIRSPV
SGRVGIRTVDEGNFLRTSDAQGLFSVTQIDPIAVEFSLPQQMLPTLQGLIAAPTQASVDA
YLGADTDGQTGDLLGEGHLSLIDNQISSTTGTLRAKAEFNNASQRLWPGQLVTIKIQTAL
DKNALVVPPTVVQRGLDSHFVYRVNGDKVDVVPVQVTYQDSDVNIIKGVQAGDVLVSDGQ
SRLKAGAQVEVLKEPPQVIQTVDAKVQP