Protein Info for GFF923 in Sphingobium sp. HT1-2

Annotation: 4-hydroxybutyrate coenzyme A transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02550: AcetylCoA_hydro
  • amino acids 7 to 158 (152 residues), 47.3 bits, E=2.6e-16
• PF13336: AcetylCoA_hyd_C
  • amino acids 256 to 407 (152 residues), 181.1 bits, E=1.4e-57

Best Hits

Swiss-Prot: 40% identical to CAT2_CLOK5: 4-hydroxybutyrate coenzyme A transferase (cat2) from Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)

KEGG orthology group: K01067, acetyl-CoA hydrolase [EC: 3.1.2.1] (inferred from 47% identity to reh:H16_B1368)

MetaCyc: 42% identical to butanoyl-CoA:acetate CoA-transferase (Clostridium kluyveri)
Propionate CoA-transferase. [EC: 2.8.3.1, 2.8.3.8]

Predicted SEED Role

"4-hydroxybutyrate coenzyme A transferase"

MetaCyc Pathways

• acetyl-CoA fermentation to butanoate (5/7 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• 1,2-propanediol biosynthesis from lactate (engineered) (2/6 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
• succinate fermentation to butanoate (2/7 steps found)
• gallate degradation III (anaerobic) (4/11 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Propanoate metabolism
• Pyruvate metabolism
• Styrene degradation

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.1

Use Curated BLAST to search for 2.8.3.1 or 2.8.3.8 or 3.1.2.1

Protein Sequence (415 amino acids)

>GFF923 4-hydroxybutyrate coenzyme A transferase (Sphingobium sp. HT1-2)
VIDLTPYLRRGDRIVFGQACGEPVSLVSALIEQGAGIGDLSAFIATSFSGLFAPESADSF
RLSSMGAIGALRSMTKAGKLQVIPIHVSQVAPMITAGIIGCDVAMIQLSPADADGNHSCG
LIGDYVRAAVDKARVVIAEINEAVPYVPGELIPASAIDVAIPVSRPPVEVAPAKISPTDE
AIARHCAAYIGDGSVIQTGVGAVPDAILRLLGDRKDLGVHSGMLGDGLVELAQAGVLTNA
RKEIDTGVSINGALIGTRRLYDWAHNNPAIRMCATSYTHDAAVLGRLSRLVTINSALEVD
LTGQVNAEQSGAAYLGGTGGQVDFVRAGARSPGGCSIIALSASAKGGSLSKIVPSLSGPV
TTARSDVDIIVTEYGAAELKGQTLAERTRRLIAIADPAFREELERAAHIIQQRGY