Protein Info for PS417_04645 in Pseudomonas simiae WCS417

Annotation: UDP-N-acetylmuramate--alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 18 to 467 (450 residues), 559.9 bits, E=2.4e-172
• PF01225: Mur_ligase
  • amino acids 18 to 116 (99 residues), 108 bits, E=3.9e-35
• PF08245: Mur_ligase_M
  • amino acids 121 to 301 (181 residues), 95 bits, E=9.4e-31
• PF02875: Mur_ligase_C
  • amino acids 321 to 410 (90 residues), 56.2 bits, E=5.4e-19

Best Hits

Swiss-Prot: 98% identical to MURC_PSEFS: UDP-N-acetylmuramate--L-alanine ligase (murC) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 98% identity to pfs:PFLU0948)

MetaCyc: 62% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Sequence Analysis Tools

See A0A1N7TW86 at UniProt or InterPro

Protein Sequence (481 amino acids)

>PS417_04645 UDP-N-acetylmuramate--alanine ligase (Pseudomonas simiae WCS417)
MVENQKAMPQPEMRRIRRIHFVGIGGVGMCGIAEVLLNLGYQVSGSDLKESPVTERLKTF
GAQIFIGHRAENAAEADVLVVSSAVNTSNPEVATALERRIPVVPRAEMLAELMRYRHGIA
VAGTHGKTTTTSLIASVFAAGGLDPTFVIGGRLNAAGTNAQLGTSRYLIAEADESDASFL
HLQPLVAVVTNIDEDHMATYDGDFNKLKKTFVEFLHNLPFYGLAVVCLDDPVVREILPLV
KRPTVTYGFSEDADVRAINVRQEGMQTFFTVLRPDREPLDVSVNMPGNHNVLNSLATICI
ATDEGVSDEAIVEGLSRFAGVGRRFQVYGQLPVDGGDVMLVDDYGHHPTEVAAVIKAVRG
GWPERRLVMVYQPHRYSRTRDLYDDFVNVLADANVLLLMEVYPAGEEPIPGADSRKLCNS
IRQRGQLDPIYIERGVDLAPIVKPLLRAGDILLCQGAGDIGGLAPKLLASPLFAAAKGKS
K