Protein Info for PS417_04620 in Pseudomonas simiae WCS417

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 26 to 72 (47 residues), 30.7 bits, 5.1e-11
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 30 to 448 (419 residues), 463.7 bits, E=2.9e-143
• PF08245: Mur_ligase_M
  • amino acids 107 to 296 (190 residues), 160.1 bits, E=1.1e-50
• PF02875: Mur_ligase_C
  • amino acids 326 to 437 (112 residues), 61.2 bits, E=2.7e-20

Best Hits

Swiss-Prot: 45% identical to MURF_ECOLI: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase (murF) from Escherichia coli (strain K12)

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 95% identity to pfs:PFLU0943)

MetaCyc: 45% identical to D-alanyl-D-alanine-adding enzyme (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase. [EC: 6.3.2.10]

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (12/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

See A0A1N7TUY2 at UniProt or InterPro

Protein Sequence (456 amino acids)

>PS417_04620 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (Pseudomonas simiae WCS417)
MLKAMKFSELTQALSARVLSSDCSFDGVSIDSRNIKPGQLFVALTGPRFDGHDYLNEVAA
KGAVGALVQRAVADSTLPQLLVADTRLALGQLGALNRAAFTNPVAAITGSSGKTTVKELL
AGVLRTRGPVHATRGNLNNDFGAPLTLLELAPEHTAAVIELGASRVGEIAYTVALTKPHV
VVINNAGTAHVGEFGGPEKIVEAKGEILEGLDASGIAVLNLDDKAFETWRVRAAGRKVLT
FAVLNAAADFHASNITVDARGCPSFTLHTPQGNEHVQLNLLGNHNVANALAAAAAAYALG
VSLFGIATGLGAVQPVKGRTVAQLATNGMRVIDDTYNANQSSICAAIDLLKSFEGRKVLV
LGDIAELGDWAEQSHREVGAYAAGKVDALYAVGPNMAHAVSVFGPGARHFVTQAELIQAL
TAAEHDKHTTILIKGSRSAVMENVVAALCGSSTEKH