Protein Info for GFF91 in Xanthobacter sp. DMC5
Annotation: hypothetical protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to ALF1_XANCP: Probable fructose-bisphosphate aldolase class 1 (XCC3185) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
KEGG orthology group: K01623, fructose-bisphosphate aldolase, class I [EC: 4.1.2.13] (inferred from 90% identity to xau:Xaut_3077)MetaCyc: 53% identical to cytosolic fructose-bisphosphate aldolase (Spinacia oleracea)
Fructose-bisphosphate aldolase. [EC: 4.1.2.13]; 4.1.2.13 [EC: 4.1.2.13]
Predicted SEED Role
"Fructose-bisphosphate aldolase class I (EC 4.1.2.13)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis (EC 4.1.2.13)
MetaCyc Pathways
- Calvin-Benson-Bassham cycle (12/13 steps found)
- gluconeogenesis I (12/13 steps found)
- formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
- superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
- ethene biosynthesis V (engineered) (20/25 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
- homolactic fermentation (10/12 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
- superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
- glycolysis III (from glucose) (9/11 steps found)
- oxygenic photosynthesis (13/17 steps found)
- superpathway of glycolysis and the Entner-Doudoroff pathway (13/17 steps found)
- pentose phosphate pathway (non-oxidative branch) II (5/6 steps found)
- sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
- superpathway of anaerobic sucrose degradation (14/19 steps found)
- glycolysis II (from fructose 6-phosphate) (8/11 steps found)
- glycolysis IV (7/10 steps found)
- glycolysis I (from glucose 6-phosphate) (9/13 steps found)
- sedoheptulose bisphosphate bypass (1/2 steps found)
- formaldehyde assimilation II (assimilatory RuMP Cycle) (6/9 steps found)
- gluconeogenesis III (8/12 steps found)
- glycolysis VI (from fructose) (7/11 steps found)
- glycolysis V (Pyrococcus) (6/10 steps found)
- sucrose degradation V (sucrose α-glucosidase) (2/5 steps found)
- hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
- 1,3-propanediol biosynthesis (engineered) (4/9 steps found)
- gluconeogenesis II (Methanobacterium thermoautotrophicum) (10/18 steps found)
- superpathway of hexitol degradation (bacteria) (9/18 steps found)
- superpathway of N-acetylneuraminate degradation (11/22 steps found)
- Methanobacterium thermoautotrophicum biosynthetic metabolism (25/56 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Fructose and mannose metabolism
- Glycolysis / Gluconeogenesis
- Pentose phosphate pathway
Isozymes
Compare fitness of predicted isozymes for: 4.1.2.13
Use Curated BLAST to search for 4.1.2.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (340 amino acids)
>GFF91 hypothetical protein (Xanthobacter sp. DMC5) MTAELDAIAQKMVADGKGILAADESGGTIKKRFDAIGVESTEENRRDYRELMFRSQGMDY ISGVILYDETIRQKAKDGTPLVSVIEKSGAIPGIKVDAGAKPQPFCPGETITEGLDGLAG RLKEYYDLGARFAKWRAVIDIDTSKHIPSYTSLRANAHALARYAALCQEAKIVPIVEPEV LMDGAHDIDTCYRVTEWALKTVFEELYYQRVRLEGMVLKPNMIVSGKKSPAQASVAEVAE KTVRVLKACVPTAVPGIAFLSGGQSDEDATAHLDAMVKLGGLPWKLTYSYGRALQAAPQK AWSGKPENVAKAQAAFAHRAHMNSLAALGRWSAEEERKAA