Protein Info for GFF90 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
  • amino acids 9 to 489 (481 residues), 646.1 bits, E=1.7e-198
• PF00483: NTP_transferase
  • amino acids 11 to 306 (296 residues), 159.2 bits, E=4.1e-50
• PF12804: NTP_transf_3
  • amino acids 12 to 140 (129 residues), 33 bits, E=2.1e-11
• PF22640: ManC_GMP_beta-helix
  • amino acids 313 to 367 (55 residues), 60 bits, 5.2e-20
• PF01050: MannoseP_isomer
  • amino acids 371 to 485 (115 residues), 186.7 bits, E=3.7e-59
• PF07883: Cupin_2
  • amino acids 402 to 469 (68 residues), 40.5 bits, E=5.5e-14
• PF02311: AraC_binding
  • amino acids 415 to 472 (58 residues), 21.2 bits, 6e-08

Best Hits

KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 64% identity to adn:Alide_0735)

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• GDP-mannose biosynthesis (3/4 steps found)
• β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
• D-mannose degradation I (1/2 steps found)
• D-mannose degradation II (1/2 steps found)
• mannitol degradation II (2/4 steps found)
• mannitol biosynthesis (1/3 steps found)
• 1,5-anhydrofructose degradation (2/5 steps found)
• superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (8/14 steps found)
• β-(1,4)-mannan degradation (2/7 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.22, 5.3.1.8

Use Curated BLAST to search for 2.7.7.22 or 5.3.1.8

Protein Sequence (497 amino acids)

>GFF90 Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VNTMPSTSIVPVVLSGGSGSRLWPLSRDQYPKQLLALGGAESLLQATLRRAAGLPGVAQA
LVVCGEDYRFVIAEQLRQIGSEGRIVLEPVGRNTAPAMAVAAHAVGAGDDDPLMLVMPAD
HLIPDATAFARAVAAAAPLAMAGAMVTFGVVPTAPETGYGYIQRGPAFALAADGTPVWSI
ARFLEKPDEATAERLVREGECLWNAGLFMVRASVWLKALRHCRPDIATAVSTTWDTRRSD
SDFIRLPMAAFAACPSDSIDYAVMERLVVGGARDPALPHGVVVPLAAGWSDVGAWNSVWQ
CLPKDEAGNAVQGDVVLRDARNTLVMAERRLVTCCGVDDLVVVETADAVLVVHKDRAQDV
KQIVEQLRREGRPESKAHRKIHRPWGWYDSVDAGARFQVKRIVVKPGAALSLQMHHHRAE
HWIVVKGTAQVTCDDREFLLTENESTFIPLGKTHRLENPGRFDLEMIEVQSGAYLGEDDI
VRFDDVYGRVPTVPALR