Protein Info for GFF89 in Xanthobacter sp. DMC5

Annotation: Glyceraldehyde-3-phosphate dehydrogenase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00044: Gp_dh_N
  • amino acids 3 to 104 (102 residues), 122.9 bits, E=6.1e-40
• TIGR01534: glyceraldehyde-3-phosphate dehydrogenase, type I
  • amino acids 4 to 326 (323 residues), 424.9 bits, E=1.1e-131
• PF02800: Gp_dh_C
  • amino acids 158 to 314 (157 residues), 217.9 bits, E=5.4e-69

Best Hits

Swiss-Prot: 98% identical to G3P_XANFL: Glyceraldehyde-3-phosphate dehydrogenase (gap) from Xanthobacter flavus

KEGG orthology group: K00134, glyceraldehyde 3-phosphate dehydrogenase [EC: 1.2.1.12] (inferred from 93% identity to xau:Xaut_3075)

MetaCyc: 53% identical to chloroplastic glyceraldehyde 3-phosphate dehydrogenase A (Arabidopsis thaliana col)
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating). [EC: 1.2.1.13]; Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating). [EC: 1.2.1.13, 1.2.1.12]

Predicted SEED Role

"NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) / NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12)" in subsystem Calvin-Benson cycle or Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Pyridoxin (Vitamin B6) Biosynthesis or Redox-dependent regulation of nucleus processes (EC 1.2.1.12, EC 1.2.1.13)

MetaCyc Pathways

• Calvin-Benson-Bassham cycle (12/13 steps found)
• gluconeogenesis I (12/13 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (11/12 steps found)
• Bifidobacterium shunt (13/15 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• ethene biosynthesis V (engineered) (20/25 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• Entner-Doudoroff pathway I (8/9 steps found)
• homolactic fermentation (10/12 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• glycolysis III (from glucose) (9/11 steps found)
• pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
• oxygenic photosynthesis (13/17 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (13/17 steps found)
• sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• glycolysis II (from fructose 6-phosphate) (8/11 steps found)
• heterolactic fermentation (13/18 steps found)
• glycolysis IV (7/10 steps found)
• glycolysis I (from glucose 6-phosphate) (9/13 steps found)
• glycerol degradation to butanol (11/16 steps found)
• gluconeogenesis III (8/12 steps found)
• glycolysis VI (from fructose) (7/11 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (12/19 steps found)
• superpathway of hexitol degradation (bacteria) (9/18 steps found)
• superpathway of N-acetylneuraminate degradation (11/22 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Glycolysis / Gluconeogenesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.12 or 1.2.1.13

Protein Sequence (335 amino acids)

>GFF89 Glyceraldehyde-3-phosphate dehydrogenase 1 (Xanthobacter sp. DMC5)
MSVKVAINGFGRIGRNVLRAIIESGRTDIEVVAINDLGPVETNAHLFRFDSVHGRFPGEV
KVAGDTIDVGRGPIKVTAVRNPAELPHKELGVDIALECTGIFTSRDKAAAHLAAGAKRVL
VSAPADGADLTVVFGVNHEKLTSEHLVVSNASCTTNCLAPVAKVLNDAVGIEKGFMTTIH
SYTGDQPTLDTMHKDLYRARAAAMSMIPTSTGAAKAVGLVLPELNGKLDGTSIRVPTPNV
SVIDLKFVAKRATSKEEINEAIIAAAAQELKGILGFTDQPNVSIDFNHNPNSSTFHLDQT
KVMEGTLVRVLSWYDNEWGFSNRMADTAVAMGKLG