Protein Info for GFF877 in Sphingobium sp. HT1-2
Annotation: Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ Cob(I)alamin adenosyltransferase (EC 2.5.1.17), clustered with cobalamin synthesis
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 58% identical to COBO_SINSX: Corrinoid adenosyltransferase (cobO) from Sinorhizobium sp.
KEGG orthology group: K00798, cob(I)alamin adenosyltransferase [EC: 2.5.1.17] (inferred from 87% identity to sjp:SJA_C2-03080)MetaCyc: 58% identical to CobO (Pseudomonas denitrificans (nom. rej.))
Cob(I)yrinic acid a,c-diamide adenosyltransferase. [EC: 2.5.1.17]
Predicted SEED Role
"Cob(I)alamin adenosyltransferase (EC 2.5.1.17)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis (EC 2.5.1.17)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide I (8/8 steps found)
- adenosylcobinamide-GDP biosynthesis from cobyrinate a,c-diamide (6/6 steps found)
- superpathway of adenosylcobalamin salvage from cobinamide II (8/9 steps found)
- adenosylcobalamin salvage from cobalamin (5/5 steps found)
- adenosylcobinamide-GDP salvage from cobinamide I (5/5 steps found)
- adenosylcobinamide-GDP salvage from cobinamide II (5/6 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (25/36 steps found)
- cobalamin salvage (eukaryotic) (4/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.17
Use Curated BLAST to search for 2.5.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (201 amino acids)
>GFF877 Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ Cob(I)alamin adenosyltransferase (EC 2.5.1.17), clustered with cobalamin synthesis (Sphingobium sp. HT1-2) MVERTDEQHAEKMKKKQAAHDKIMATKTQEKGLLIVHTGKGKGKTTAALGMVVRMIGHGK KVGVVQFVKGAMTTGEKAVFDAFPEQVEFKPMGEGFTWNTQDRSRDIALAREAWDEVKRM VADPDYAMVLADELNIVLRYDYLPLDEVLAVLTGRDEMKHVVVTGRNAPEALIEAADLVT EMTMIKHPFRSGVKAQAGIEY