Protein Info for GFF876 in Sphingobium sp. HT1-2

Annotation: Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 211 TIGR02476: 5,6-dimethylbenzimidazole synthase" amino acids 4 to 202 (199 residues), 247.2 bits, see alignment E=5.2e-78 PF00881: Nitroreductase" amino acids 17 to 183 (167 residues), 99.7 bits, see alignment E=1.1e-32

Best Hits

Swiss-Prot: 52% identical to BLUB_RHOCB: 5,6-dimethylbenzimidazole synthase (bluB) from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)

KEGG orthology group: K04719, 5,6-dimethylbenzimidazole synthase [EC: 1.14.99.40] (inferred from 59% identity to nar:Saro_0330)

Predicted SEED Role

"Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.99.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (211 amino acids)

>GFF876 Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family (Sphingobium sp. HT1-2)
MQAFDPDFCATLTELLTWRRDVRHFRTDPLDEAMVLELLTLAQLAPSVGNSQPWRFVRVR
TPPLRAALADHVDAERERATSQITDATRHDAYRALKLHGLGEAPEIIAVFCDDATQAGAG
LGAATMPQTRLHSVVLAIHTLWLAARARGLALGWVSILDPAFVVRTLAVPDDWTFAALLC
LGHPEAVADTPELARLGWQARLDPLAQISER