Protein Info for GFF862 in Xanthobacter sp. DMC5

Annotation: Valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 929 transmembrane" amino acids 522 to 540 (19 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 3 to 908 (906 residues), 1086.7 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 15 to 611 (597 residues), 511.3 bits, see alignment E=4.6e-157 PF09334: tRNA-synt_1g" amino acids 45 to 93 (49 residues), 28.5 bits, see alignment 1.7e-10 amino acids 557 to 617 (61 residues), 30.7 bits, see alignment 3.8e-11 PF13603: tRNA-synt_1_2" amino acids 245 to 304 (60 residues), 27.2 bits, see alignment 6.6e-10 PF08264: Anticodon_1" amino acids 654 to 803 (150 residues), 117.8 bits, see alignment E=9.7e-38 PF10458: Val_tRNA-synt_C" amino acids 861 to 925 (65 residues), 62 bits, see alignment 1.3e-20

Best Hits

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 85% identity to azc:AZC_2815)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (929 amino acids)

>GFF862 Valine--tRNA ligase (Xanthobacter sp. DMC5)
MLEKVFDPAAVEERIADRWENAGAFRCGRPERKDAEGFSIVIPPPNVTGSLHMGHALNNT
LQDVLARFERMRGKDVLWQPGTDHAGIATQMVVERQLAAQKLPGRRDLGREAFIEKVWAW
KAESGGTIVNQLKKLGASCDWSRERFTMDEGLSRAVLKVFVQLYREGLIYKDKRLVNWDP
KLITAISDLEVLQVEVKGNLWHLRYPIEGEKDRFIVVATTRPETMLGDTAVAVHPDDERY
RDLIGKHVILPLVGRRIPIVADEYSDPGKGSGAVKITPAHDFNDFDVGRRHDLPMINVLD
AEARIDPQGIQDDYAERPEAYVDLPDGAGVLTHLAGMDRFAARKHIVELLTSLELLEKIE
PHTYMVPHGDRSNVVIEPWLTDQWYVDAKTLAQPALAAVREGRTTFVPKNWEKTYFEWLE
NIQPWCISRQLWWGHQIPAWYDPFGNVFVAEDEDQAFEEALAHNVGNESLSPEEAQALID
DADKRAQFLTRDEDVLDTWFSSALWPFSTMGWPDETAELKKFYPTSVLVTGFDIIFFWVA
RMMMMGLHFMDGEVPFKDVYIHALVRDEKGAKMSKSKGNVIDPLDLVEKYGADALRFTLA
AMAAQGRDIKLATSRVEGYRNFATKLWNAVRFAQMNGCVRTDGFDPAKVEGTLNRWIIGE
AARASSEVSEAILAYRFNEAAGAVYRFIWNVVCDWHLELAKPVLSGPDGAAKDETRAATA
YVLDVAMGLLHPFMPFLTEELWAETGKEGPARTSLLALAPWPDLTGLEAPDAEAEVGWVV
DLVTEIRSVRAEMNVPAGAQVPLVLVQPGAETTARVAAWDDAIRRLARLSDVSVSDQVPA
SSVQMVVRGEVVALPLAGVVDLGAELARLRKEEGKLDQEVARIDAKLSNESFVARAPEEV
VEAEREKREEYILRKEKVRAAIGQLSAGA