Protein Info for GFF855 in Sphingobium sp. HT1-2

Annotation: Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 PF13450: NAD_binding_8" amino acids 11 to 64 (54 residues), 22.2 bits, see alignment 2.2e-08 PF00732: GMC_oxred_N" amino acids 71 to 336 (266 residues), 45.2 bits, see alignment E=1.6e-15 PF05199: GMC_oxred_C" amino acids 424 to 543 (120 residues), 73.3 bits, see alignment E=4.5e-24

Best Hits

KEGG orthology group: None (inferred from 49% identity to rbi:RB2501_02785)

MetaCyc: 50% identical to D-glucoside-3-dehydrogenase catalytic subunit (Agrobacterium fabrum)
GLUCOSIDE-3-DEHYDROGENASE-RXN [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]; 1.1.2.11 [EC: 1.1.2.11]

Predicted SEED Role

"Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site" in subsystem Respiratory dehydrogenases 1

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.2.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (560 amino acids)

>GFF855 Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site (Sphingobium sp. HT1-2)
MASTDRYDAIVIGSGISGGWAAKELTEKGLRVLMLDRGHMVEHGEDYTYDGKPAYEVPAR
NIMPDPLKQSDYFIAQHGYVAPSNQAFYNNDRLNPYDYGAGSKFYWIRPAAVGGKSLIWG
RWSFRWSPADFEANKREGIGGDWPIRYEDLVPWYSYVEKYIGVSGSRENLAYLPDSEFQP
PMPMNIAEKWMKQRLETQFPGRKMINARLSNMTEDKPEQNRTKCQLRNQCGNGCSFGAYF
STQAVTLPAARATGRLTLRSDAVVTNLDYDPATKKVSSVRFVDAKTGQAETVTARLVFLC
ASALASTQILMNSRPAGSGKSHFDSSGTLGRYVMDHIFRVGVKGDIPGMEEFIEYGRRPG
AIYVPRFRNNGDSEDVGFKRGYGYQGGAYREPSKPVGFGASMKQGMRKYGGWKFQMGAFG
ECLPYEDNYVALSPDKTDRFGMPLMQFNVTFRDNEMRMMADAREQGEKMLRAAGLTNVTS
SVREHVPGDAIHEMGGARMGADPRASVLNQWSQAHDASNLFVTDGAQMASISCVNPSLTF
MALTARAVDHAVKEMKAGRV