Protein Info for GFF846 in Xanthobacter sp. DMC5

Annotation: Adaptive-response sensory-kinase SasA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 743 transmembrane" amino acids 21 to 46 (26 residues), see Phobius details amino acids 55 to 75 (21 residues), see Phobius details amino acids 96 to 119 (24 residues), see Phobius details amino acids 293 to 317 (25 residues), see Phobius details PF19312: NtrY_N" amino acids 27 to 303 (277 residues), 120 bits, see alignment E=4.6e-38 PF00672: HAMP" amino acids 316 to 367 (52 residues), 43.7 bits, see alignment 9.9e-15 PF00989: PAS" amino acids 387 to 490 (104 residues), 55.1 bits, see alignment E=2.5e-18 TIGR00229: PAS domain S-box protein" amino acids 389 to 498 (110 residues), 28.6 bits, see alignment E=6.8e-11 PF08448: PAS_4" amino acids 394 to 492 (99 residues), 30.6 bits, see alignment E=1.2e-10 PF13426: PAS_9" amino acids 397 to 492 (96 residues), 28.5 bits, see alignment E=5.6e-10 PF00512: HisKA" amino acids 504 to 569 (66 residues), 40.6 bits, see alignment E=7.5e-14 PF02518: HATPase_c" amino acids 612 to 722 (111 residues), 75.5 bits, see alignment E=1.6e-24

Best Hits

Swiss-Prot: 75% identical to NTRY_AZOC5: Nitrogen regulation protein NtrY (ntrY) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K13598, two-component system, NtrC family, nitrogen regulation sensor histidine kinase NtrY [EC: 2.7.13.3] (inferred from 84% identity to xau:Xaut_4398)

Predicted SEED Role

"Nitrogen regulation protein NtrY (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (743 amino acids)

>GFF846 Adaptive-response sensory-kinase SasA (Xanthobacter sp. DMC5)
MNDEPVGYAARDVRPRRSLFARLFAPVVVVLSLVLAIATFAVIMGLTSIVPTPTVTIAAL
ASSAVAAVALLGIIGREVWRIWQARARGRAASRLHVRIIGLFAIVAVVPAVVVALVASFT
LDRTLDRYFSERTQEIVGSASSVAQTYVREHALSIRGDALAMANDLSQQKNLYDTDIDKF
RQVLTAQAALRNVPGAQIIHRDLSVVERANLRTGREFLVPANIAINEATAEQPIIYVDAD
YVGAVVPLTGFDDLFLYVARPIDPRVLGYLKVTSDTLADYRALEQRRFSVQLAFAAMYSI
VTLIVLLSAVWLGVTFSKWLVAPIRRLMWAADRVAKGNLDIQVPVNRAEGDLATLGETFN
KMTGELKTQRNALVSARDQMDSRRRFTEAVLSGVGAGVIGIDADARVTILNLSAERLLGV
TEAEELGRPLAEVVPETAALLEEARGAGQRAVQGNITVQRDGRERVFAMRVTTEQSGAEA
HGWVVTLDDITALITAQRTSAWADVARRIAHEIKNPLTPIQLSAERLKRKYGRHITQDRD
VFDQCTDTIIRQVGDIGRMVDEFSSFARMPKPVVDAQDLAETVRQAVFLMRVGHPDVTFE
AELPAAQPARFDRRLISQALTNILKNAAEAIEAVPDAERGKGRIRVRVTAAGEDLLIDVI
DNGAGLPREKRSRLLEPYVTTREKGTGLGLAIVGKIMEEHGGGIELNDSPEGRGAWIRLR
MRADGLPPAGAGGAQPVPQAGRA