Protein Info for PS417_04295 in Pseudomonas simiae WCS417

Annotation: peptidase PmbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 248 to 262 (15 residues), see Phobius details PF01523: PmbA_TldD_1st" amino acids 34 to 98 (65 residues), 46.1 bits, see alignment E=6.8e-16 PF19290: PmbA_TldD_2nd" amino acids 126 to 232 (107 residues), 76.1 bits, see alignment E=4.4e-25 PF19289: PmbA_TldD_3rd" amino acids 240 to 447 (208 residues), 228.2 bits, see alignment E=1.1e-71

Best Hits

Swiss-Prot: 49% identical to PMBA_HAEIN: Metalloprotease PmbA homolog (pmbA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03592, PmbA protein (inferred from 98% identity to pfs:PFLU0872)

Predicted SEED Role

"TldE protein, part of TldE/TldD proteolytic complex"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U246 at UniProt or InterPro

Protein Sequence (448 amino acids)

>PS417_04295 peptidase PmbA (Pseudomonas simiae WCS417)
MSAAQSVGPQALPALQEQVEQILAEAKRQGASACEVAVSLEQGLSTSVRQREVETVEFNR
DQGFGITLYMGQRKGSASTSASGPDAIRETVAAALAIAKHTSEDESSGLADKALMAKDLQ
DFDLFHAWDITPEQAIEKALICEAAAFDADPRIKNADGTTLSTHQGCRVYGNSHGFIGGY
ASTRHSLSCVMIAEADGQMQRDYWYDVNRQGELLADAVSIGQRAAQRAASRLGARPVPTC
EVPVLFSAELAGGLFGSFLGAISGGNLYRKSSFLEGSIGQKLFPEWLTIDERPHLMRAMG
STSFDGDGLATYAKPFVEKGELVSYVLGTYAGRKLGLPSTANSGGVHNLFVTHGDEDQAA
LLRRMGRGLLVTELMGHGLNMVTGDYSRGAAGFWVENGEIQFAVQEVTIAGNMRDMFKQI
VAVGNDLELRSNIRTGSVLIERMTVAGS