Protein Info for GFF842 in Xanthobacter sp. DMC5

Annotation: D-alanine aminotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 38% identical to DAAA_BACYM: D-alanine aminotransferase (dat) from Bacillus sp. (strain YM-1)

KEGG orthology group: K00824, D-alanine transaminase [EC: 2.6.1.21] (inferred from 84% identity to xau:Xaut_4394)

Predicted SEED Role

"D-alanine aminotransferase (EC 2.6.1.21)" in subsystem Pyruvate Alanine Serine Interconversions (EC 2.6.1.21)

MetaCyc Pathways

D-alanine degradation (1/1 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (285 amino acids)

>GFF842 D-alanine aminotransferase (Xanthobacter sp. DMC5)
MSRIAYVNGRYLPHRDASVHVEDRGYQFADGVYEVCEVLGGRLVDERRHLDRLDRSLREL
FIAAPMSRAALSAVLREVVRRNGVRDGMVYLQVTRGVARRDHAFPAPDIAPSVVVTARRT
DREAQEALARKGVAVITVPENRWPRVDIKSVSLLPNVLAKQQAKEAGAREAWFVDADGYV
TEGASTNAWIVTADRTIVTRPADSGILKGITRTVAFEVAADLGYQIEERPFTVAEAHAAD
EAFISAATTVIMPVVRIDGTPVGTGVPGPVATALRLKFHDFSEVA