Protein Info for GFF822 in Sphingobium sp. HT1-2

Annotation: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 279 PF14805: THDPS_N_2" amino acids 5 to 69 (65 residues), 91.2 bits, see alignment E=5.7e-30 TIGR00965: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase" amino acids 5 to 278 (274 residues), 422.9 bits, see alignment E=2.9e-131 PF14602: Hexapep_2" amino acids 177 to 211 (35 residues), 50.1 bits, see alignment 2.8e-17 PF00132: Hexapep" amino acids 178 to 211 (34 residues), 30.1 bits, see alignment 4.1e-11

Best Hits

Swiss-Prot: 74% identical to DAPD_METNO: 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) from Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)

KEGG orthology group: K00674, 2,3,4,5-tetrahydropyridine-2-carboxylate N-succinyltransferase [EC: 2.3.1.117] (inferred from 95% identity to sjp:SJA_C1-01570)

MetaCyc: 65% identical to tetrahydrodipicolinate succinylase (Escherichia coli K-12 substr. MG1655)
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase. [EC: 2.3.1.117]

Predicted SEED Role

"2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117)" in subsystem Lysine Biosynthesis DAP Pathway (EC 2.3.1.117)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.117

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (279 amino acids)

>GFF822 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (EC 2.3.1.117) (Sphingobium sp. HT1-2)
MSQDLIAIIDAAWEDRANINLSTQGDVRQAVDKALALLDKGELRVAEPTEAGWQVNQWAK
KAVLLSFRLNDNVMIDNGPGAGHWWDKVPSKFAGWDEAAFRAAGFRAVPGSFARAGSHIA
KNVILMPSFVNIGAFVDEGTMVDTWVTVGSCAQIGKNVHLSGGVGIGGVLEPLQADPVII
EDDCFIGARSEVVEGVRIGKGAVLSMGVFIGQSTKIIDRATGEVFMGEVPPYSVVVPGSL
PGKPLPDGTPGPSLYCAVIVKRVDAQTRSKTGINELLRD