Protein Info for GFF818 in Sphingobium sp. HT1-2

Annotation: Phosphoserine aminotransferase (EC 2.6.1.52)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 60% identical to SERC_METAC: Phosphoserine aminotransferase (serC) from Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)

KEGG orthology group: K00831, phosphoserine aminotransferase [EC: 2.6.1.52] (inferred from 92% identity to sjp:SJA_C1-01850)

MetaCyc: 60% identical to phosphoserine aminotransferase monomer (Methanosarcina acetivorans)
Phosphoserine transaminase. [EC: 2.6.1.52]; Aspartate transaminase. [EC: 2.6.1.52, 2.6.1.1]

Predicted SEED Role

"Phosphoserine aminotransferase (EC 2.6.1.52)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 2.6.1.52)

MetaCyc Pathways

aspartate superpathway (25/25 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (18/18 steps found)
superpathway of aromatic amino acid biosynthesis (18/18 steps found)
superpathway of L-isoleucine biosynthesis I (13/13 steps found)
superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
superpathway of L-phenylalanine biosynthesis (10/10 steps found)
superpathway of L-tyrosine biosynthesis (10/10 steps found)
superpathway of L-methionine biosynthesis (transsulfuration) (9/9 steps found)
superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
superpathway of L-threonine biosynthesis (6/6 steps found)
L-tyrosine degradation I (5/5 steps found)
superpathway of L-serine and glycine biosynthesis I (4/4 steps found)
pyridoxal 5'-phosphate biosynthesis I (6/7 steps found)
L-phenylalanine biosynthesis I (3/3 steps found)
L-serine biosynthesis I (3/3 steps found)
L-tyrosine biosynthesis I (3/3 steps found)
TCA cycle VIII (Chlamydia) (5/6 steps found)
malate/L-aspartate shuttle pathway (2/2 steps found)
3-(4-hydroxyphenyl)pyruvate biosynthesis (1/1 steps found)
L-aspartate biosynthesis (1/1 steps found)
L-aspartate degradation I (1/1 steps found)
anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
L-asparagine degradation III (mammalian) (2/3 steps found)
L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
L-glutamate degradation II (1/2 steps found)
L-tryptophan degradation IV (via indole-3-lactate) (1/2 steps found)
L-tyrosine degradation II (1/2 steps found)
atromentin biosynthesis (1/2 steps found)
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (8/12 steps found)
L-phenylalanine degradation III (2/4 steps found)
L-tyrosine degradation III (2/4 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (2/4 steps found)
C4 photosynthetic carbon assimilation cycle, NAD-ME type (7/11 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (9/14 steps found)
(R)-cysteate degradation (1/3 steps found)
L-phenylalanine degradation II (anaerobic) (1/3 steps found)
L-tyrosine degradation IV (to 4-methylphenol) (1/3 steps found)
indole-3-acetate biosynthesis VI (bacteria) (1/3 steps found)
sulfolactate degradation III (1/3 steps found)
superpathway of chorismate metabolism (41/59 steps found)
superpathway of plastoquinol biosynthesis (2/5 steps found)
L-tryptophan degradation VIII (to tryptophol) (1/4 steps found)
superpathway of sulfolactate degradation (2/6 steps found)
4-hydroxybenzoate biosynthesis I (eukaryotes) (1/5 steps found)
L-phenylalanine degradation VI (reductive Stickland reaction) (1/5 steps found)
L-tryptophan degradation XIII (reductive Stickland reaction) (1/5 steps found)
L-tyrosine degradation V (reductive Stickland reaction) (1/5 steps found)
coenzyme M biosynthesis II (1/6 steps found)
L-phenylalanine degradation IV (mammalian, via side chain) (2/9 steps found)
rosmarinic acid biosynthesis I (2/10 steps found)
indole-3-acetate biosynthesis II (3/12 steps found)
(S)-reticuline biosynthesis I (1/11 steps found)
superpathway of rosmarinic acid biosynthesis (3/14 steps found)
anaerobic aromatic compound degradation (Thauera aromatica) (2/27 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (19/56 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.1

Use Curated BLAST to search for 2.6.1.1 or 2.6.1.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (387 amino acids)

>GFF818 Phosphoserine aminotransferase (EC 2.6.1.52) (Sphingobium sp. HT1-2)
MTDLTAVDATIAPVAKPANRPARPFFSSGPCAKPPGWDAAKLATESLGRSHRAKIGKTRL
AYSIDLMRELLNLPDTHRIGIVPGSDTGAFEMAMWTMLGARPVTTLAWESFGEGWVTDAA
KQLKLDPTVIRADYGQLPDLTTVDFSNDVLFTWNGTTSGVRVPNADWIPADREGLTFADA
TSAVFAYEIDWTKIDVATFSWQKVLGGEGGHGVLILGPRAVERLENHTPAWPLPKVFRLM
AKGKLAEGVFKGETINTPSMLAVEDAIFSLEWGKSLGGLNGLIARSNANAAALGKIVAER
DWLGHLAVDEASRSTTSVCLTVEGADEAFIKAFAGLLEKEGAAYDVAGYRDAPAGLRIWC
GATVETADIEALGPWLDWAYAQTKAAA