Protein Info for GFF814 in Sphingobium sp. HT1-2

Annotation: Adenylosuccinate synthetase (EC 6.3.4.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00184: adenylosuccinate synthase
  • amino acids 6 to 427 (422 residues), 510.6 bits, E=1.7e-157
• PF00709: Adenylsucc_synt
  • amino acids 6 to 421 (416 residues), 576.7 bits, E=1.3e-177

Best Hits

Swiss-Prot: 84% identical to PURA_SPHWW: Adenylosuccinate synthetase (purA) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01939, adenylosuccinate synthase [EC: 6.3.4.4] (inferred from 95% identity to sjp:SJA_C1-01810)

Predicted SEED Role

"Adenylosuccinate synthetase (EC 6.3.4.4)" in subsystem CBSS-262719.3.peg.410 or Purine conversions (EC 6.3.4.4)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• superpathway of purine nucleotide salvage (11/14 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.4

Protein Sequence (429 amino acids)

>GFF814 Adenylosuccinate synthetase (EC 6.3.4.4) (Sphingobium sp. HT1-2)
MANVTVIGAQWGDEGKGKIVDWLSERADVVARFQGGHNAGHTLVVGEKVYKLSLLPSGIV
RGTLSVIGNGVVLDPWHFRDEVAKLKGQGVEITPANLQIAETCPLILPFHRDLDGLREDA
SGAGKIGTTRRGIGPAYEDKVGRRAIRVCDLAHLDDLDLQIDRLTAHHDALRAGFGEAPI
DRAQLMADLTEIAAFILPFVKPVWLTLNKAKSEGKRILFEGAQGTLLDIDHGTYPFVTSS
NTVAGTAASGTGLGPNGAGFVLGIVKAYTTRVGSGPFPTEQENDVGQRLGERGHEFGTVT
GRKRRCGWFDAVLVRQSVAVSGVTGIALTKLDVLDGFEELKICVGYKIGDQSFDYLPAHA
QDQAKAEPIYESIAGWSETTAGARSWAELPAQAIKYIRRIEELIGCPVTLVSTSPERDDT
ILVRDPFAD