Protein Info for PS417_04120 in Pseudomonas simiae WCS417

Annotation: tryptophan--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 PF00579: tRNA-synt_1b" amino acids 3 to 284 (282 residues), 179.6 bits, see alignment E=4.4e-57 TIGR00233: tryptophan--tRNA ligase" amino acids 4 to 335 (332 residues), 321.3 bits, see alignment E=3.9e-100

Best Hits

Swiss-Prot: 80% identical to SYW_PSEPK: Tryptophan--tRNA ligase (trpS) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 97% identity to pfs:PFLU0834)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U6Y8 at UniProt or InterPro

Protein Sequence (451 amino acids)

>PS417_04120 tryptophan--tRNA ligase (Pseudomonas simiae WCS417)
MTTRTRILTGITTTGTPHLGNYAGAIRPAILASQDANADSFYFLADYHALIKCDDPQRIQ
RSRMEIAATWLAGGLDVNRVTFYRQSDIPEIPELTWLLTCVAAKGLLNRAHAYKASVDKN
VEAGEDPDAGISMGLYSYPVLMAADILMFNAHKVPVGRDQIQHVEMARDIGQRFNHLFGN
GKEFFTMPEALIEESVATLPGLDGRKMSKSYDNTIPLFTSAKDMKDAISRIVTDSRAPGE
AKDPDNSHLFTLYQAFASKAQEEEFRAELLQGLGWGEAKNRLFQLLDGQLGEARERYHQL
MSRPSDMEDLLLVGANKARAVAAPFLAELREAVGLRSFVNPIAAPVATKKKAAKAARFVS
FREDDGSFRFRLLAAEGEQLLLSRNFADGKAAGAVTKQLQSGDGLDVRTEALSFSVWLDG
AAVADSAEFADEASRDAAIAALRVALTPLED