Protein Info for GFF807 in Variovorax sp. SCN45

Annotation: FIG00003370: Multicopper polyphenol oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02578: Cu-oxidase_4
  • amino acids 21 to 250 (230 residues), 246.5 bits, E=1.3e-77
• TIGR00726: YfiH family protein
  • amino acids 27 to 250 (224 residues), 171.4 bits, E=9.4e-55

Best Hits

Swiss-Prot: 49% identical to POLOX_ECOLI: Polyphenol oxidase (yfiH) from Escherichia coli (strain K12)

KEGG orthology group: K05810, conserved hypothetical protein (inferred from 81% identity to vap:Vapar_2020)

MetaCyc: 49% identical to purine nucleoside phosphorylase YfiH (Escherichia coli K-12 substr. MG1655)
Adenosine deaminase. [EC: 3.5.4.4]; S-methyl-5'-thioadenosine phosphorylase. [EC: 3.5.4.4, 2.4.2.28]; Purine-nucleoside phosphorylase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; 2.4.2.1 [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; Laccase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1, 1.10.3.2]

Predicted SEED Role

"COG1496: Uncharacterized conserved protein"

MetaCyc Pathways

• superpathway of purine nucleotide salvage (12/14 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation II (aerobic) (9/11 steps found)
• S-methyl-5'-thioadenosine degradation II (1/1 steps found)
• adenine and adenosine salvage III (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• ureide biosynthesis (5/7 steps found)
• adenine and adenosine salvage V (2/3 steps found)
• purine ribonucleosides degradation (4/6 steps found)
• adenine and adenosine salvage I (1/2 steps found)
• guanine and guanosine salvage I (1/2 steps found)
• xanthine and xanthosine salvage (1/2 steps found)
• purine deoxyribonucleosides degradation II (1/3 steps found)
• superpathway of guanine and guanosine salvage (1/3 steps found)
• purine deoxyribonucleosides degradation I (1/4 steps found)
• L-methionine salvage cycle III (6/11 steps found)
• superpathway of purine deoxyribonucleosides degradation (3/7 steps found)
• nucleoside and nucleotide degradation (archaea) (3/10 steps found)
• sesamin biosynthesis (1/8 steps found)
• arsenic detoxification (mammals) (7/17 steps found)
• justicidin B biosynthesis (1/10 steps found)
• matairesinol biosynthesis (1/10 steps found)
• salinosporamide A biosynthesis (4/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Methionine metabolism
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.4

Use Curated BLAST to search for 1.10.3.2 or 2.4.2.1 or 2.4.2.28 or 3.5.4.4

Protein Sequence (252 amino acids)

>GFF807 FIG00003370: Multicopper polyphenol oxidase (Variovorax sp. SCN45)
MDARWLVPEWPAPPNVRAVCTTRDGGVSLGRYQSLNLGDHVGDEAAHVAENRGRLRAAIE
ARPVFLQQVHGTGVVALDDEQHVVRDGTAADACTAIATGLACTIMVADCLPVLFADEAGH
RVAAAHAGWRGLAGGVLEQTARSFASGAVGKVIAWLGPCIGPKAFEVGPEVKAAFEAHAP
EAAECFRPSASAGKWLADLPALARQRLRAAGIAAVHGNDGTDDWCTVANASRFFSHRRDG
VSGRFAALVWKV