Protein Info for GFF789 in Variovorax sp. SCN45

Annotation: protease Do

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR02037: peptidase Do" amino acids 36 to 451 (416 residues), 419.2 bits, see alignment E=1e-129 PF00089: Trypsin" amino acids 84 to 241 (158 residues), 54.7 bits, see alignment E=3.9e-18 PF13365: Trypsin_2" amino acids 87 to 220 (134 residues), 122.9 bits, see alignment E=5.5e-39 PF00595: PDZ" amino acids 257 to 337 (81 residues), 35.7 bits, see alignment E=2.8e-12 amino acids 368 to 442 (75 residues), 25.9 bits, see alignment E=3.2e-09 PF13180: PDZ_2" amino acids 259 to 350 (92 residues), 42.1 bits, see alignment E=2.7e-14 PF17820: PDZ_6" amino acids 286 to 339 (54 residues), 43.7 bits, see alignment 5.5e-15 amino acids 400 to 444 (45 residues), 33.5 bits, see alignment 8.5e-12

Best Hits

KEGG orthology group: K01362, [EC: 3.4.21.-] (inferred from 67% identity to mms:mma_2365)

Predicted SEED Role

"HtrA protease/chaperone protein"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (462 amino acids)

>GFF789 protease Do (Variovorax sp. SCN45)
MGKSVPARLAALVTAAACLAPGLPLHAAMPWPASGLSQLVKAYGPAVVHIGVERPSAPAR
RFSDRWLLRVPGQLPQPQAEGDESSLGTGFIVSDDGLILTNAHVVARGTQINVKLPDRRE
FKARLVGLDAVADVALLKIDAAGLPTVRIGDPSGVEPGDGVVAIGSPYGFGNSVTAGIVS
AKGRLLPGAEYMQFLQTDVPINPGNSGGPLFNLRGEVIGINSRIYSRSGGYQGLSFAIPI
DAAMRIKDQLLSTGTVTRGRIGVSVQEVGQALADTFRLPRPAGALVNYVEPRGAADRGGL
KPGDVILSVQGREVVQAADALGFIAEQAPGENIRLRVWRERAEQMLEVRVEGFDTPRLQA
DAAQAPSAPSRLGFAVRPLAPAERQFLRVDGGLLVQRVNAAASRAGVQPGDLILALNGQP
IDSAEALVQELDVADGSAALLVQRGSTRIFVPICARAGQSSC