Protein Info for GFF785 in Sphingobium sp. HT1-2

Annotation: Ammonium transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details transmembrane" amino acids 42 to 64 (23 residues), see Phobius details amino acids 75 to 95 (21 residues), see Phobius details amino acids 140 to 161 (22 residues), see Phobius details amino acids 169 to 192 (24 residues), see Phobius details amino acids 204 to 226 (23 residues), see Phobius details amino acids 238 to 258 (21 residues), see Phobius details amino acids 270 to 290 (21 residues), see Phobius details amino acids 300 to 319 (20 residues), see Phobius details amino acids 325 to 345 (21 residues), see Phobius details amino acids 357 to 377 (21 residues), see Phobius details amino acids 396 to 419 (24 residues), see Phobius details TIGR00836: ammonium transporter" amino acids 40 to 446 (407 residues), 434.1 bits, see alignment E=2.5e-134 PF00909: Ammonium_transp" amino acids 42 to 446 (405 residues), 388.1 bits, see alignment E=2.1e-120

Best Hits

KEGG orthology group: K03320, ammonium transporter, Amt family (inferred from 80% identity to nar:Saro_0694)

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>GFF785 Ammonium transporter (Sphingobium sp. HT1-2)
MTFSKKLCGALGATGLSLFAALPAWAQDAAAPAATVDKGDVAWMMTSTILVFLMILPGLA
LFYGGLARSKNMLSVMTQIGAAACLAMLIWVMYGYSMAFGPDYTSGLSNFVSTFDKAFLK
GITPASQAATFTAGVEIPEYVFVCFQMTFAAITVALVLGSVVERIKFSAVMVFAAVWLTI
VYFPIAHMVWAASGYFFKAGALDFAGGTVVHINAGVSALVACLILGKRIGFPKEPMAPHS
LTLTAVGTGLLWAGWFGFNAGSALEANGSAALAMINTFVATASGGLFWMLIERVNGHKAS
ALGFCSGVIAGLVAVTPAAGNSGPFGAIVLGAVASIVCFYAVTVLKPKLGYDDSLDAFGI
HGIGGMIGAIGTAIVYSPALGGPGAADYEIGAKLLVQIEAVVVTIIWASIGTAIAIFIAK
AITGLRVSQEVEYEGLDLGEHGERAYNY